(S)-hidroksinitrilna lijaza
(Preusmjereno sa stranice MeHNL)
(S)-hidroksinitrilna lijaza (EC 4.1.2.47, (S)-cijanohidrin produkujuća hidroksinitrilna lijaza, (S)-oksinitrilaza, (S)-HbHNL, (S)-MeHNL, hidroksinitrilna lijaza, oksinitrilaza, HbHNL, MeHNL, (S)-selektivna hidroksinitrilna lijaza, (S)-cijanohidrin karbonil-lijaza (formira cijanid)) je enzim sa sistematskim imenom (S)-cijanohidrin lijaza (formira cijanid).[1][2][3][4][5][6][7][8][9][10] Ovaj enzim katalizuje sledeću hemijsku reakciju
| (S)-hidroksinitrilna lijaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.1.2.47 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) alifatični (S)-hidroksinitril cijanid + alifatični aldehid ili keton
- (2) aromatična (S)-hidroksinitril cijanid + aromatični aldehid
Ovaj enzim katalizuje razlaganje hidroksinitrila u cijanid i korespondirajući aldehid ili keton.
Reference uredi
- ↑ Förster, S., Roos, J., Effenberger, F., Wajant, H. and Sprauer, A. (1996). „The first recombinant hydroxynitrile lyase and its application in the synthesis of (S)-cyanohydrins”. Angew. Chem. Int. Ed. 35: 437-439.
- ↑ Bühler, H., Effenberger, F., Förster, S., Roos, J. and Wajant, H. (2003). „Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta”. Chembiochem. 4: 211-216. PMID 12616635.
- ↑ Semba, H., Dobashi, Y. and Matsui, T. (2008). „Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor”. Biosci. Biotechnol. Biochem. 72: 1457-1463. PMID 18540112.
- ↑ Avi, M., Wiedner, R.M., Griengl, H. and Schwab, H. (2008). „Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4′-oxocyclohexyl)acetonitrile as the model”. Chemistry 14: 11415-11422. PMID 19006143.
- ↑ von Langermann, J., Guterl, J.K., Pohl, M., Wajant, H. and Kragl, U. (2008). „Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values”. Bioprocess Biosyst. Eng. 31: 155-161. PMID 18204865.
- ↑ Schmidt, A., Gruber, K., Kratky, C. and Lamzin, V.S. (2008). „Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis”. J. Biol. Chem. 283: 21827-21836. PMID 18524775.
- ↑ Gartler, G., Kratky, C. and Gruber, K. (2007). „Structural determinants of the enantioselectivity of the hydroxynitrile lyase from Hevea brasiliensis”. J. Biotechnol. 129: 87-97. PMID 17250917.
- ↑ Wagner, U.G., Schall, M., Hasslacher, M., Hayn, M., Griengl, H., Schwab, H. and Kratky, C. (1996). „Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis”. Acta Crystallogr. D Biol. Crystallogr. 52: 591-593. PMID 15299689.
- ↑ Schmidt, M., Herve, S., Klempier, N. and Griengl, H. (1996). „Preparation of optically active cyanohydrins using the (S)-hydroxynitrile lyase from Hevea brasiliensis”. Tetrahedron 52: 7833-7840.
- ↑ Klempier, N. and Griengl, H. (1993). „Aliphatic (S)-cyanohydrins by enzyme catalyzed synthesis”. Tetrahedron Lett. 34: 4769-4772.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.