Triptofanska sintaza
Triptofanska sintaza (EC 4.2.1.20, L-triptofanska sintetaza, indolglicerol fosfatna aldolaza, triptofanska desmolaza, triptofanska sintetaza, L-serin hidrolijaza (dodaje indolglicerol-fosfat)) je enzim sa sistematskim imenom L-serin hidrolijaza (adding 1-C-(indol-3-il)glicerol 3-fosfat, L-triptofan and formira gliceraldehid-3-fosfat).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Triptofanska sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| |||||||||
| Triptofanska sintaza heterotetramer, Salmonella enterica | |||||||||
| Identifikatori | |||||||||
| EC broj | 4.2.1.20 | ||||||||
| CAS broj | 9014-52-2 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- L-serin + 1-C-(indol-3-il)glicerol 3-fosfat L-triptofan + gliceraldehid 3-fosfat + H2O (sveukupna reakcija)
- (1a) 1-C-(indol-3-il)glicerol 3-fosfat indol + gliceraldehid 3-fosfat
- (1b) L-serin + indol L-triptofan +H2O
Ovaj enzim je piridoksal-fosfatni protein.
Reference uredi
- ↑ Crawford, I.P. and Yanofsky, C. (1958). „On the separation of the tryptophan synthetase of Escherichia coli into two protein components”. Proc. Natl. Acad. Sci. USA 44: 1161-1170. PMID 16590328.
- ↑ Creighton, T.E. and Yanofsky, C. (1970). „Chorismate to tryptophan (Escherichia coli) - anthranilate synthetase, PR transferase, PRA isomerase, InGP synthetase, tryptophan synthetase”. Methods Enzymol. 17A: 365-380.
- ↑ Hütter, R., Niederberger, P. and DeMoss, J.A. (1986). „Tryptophan synthetic genes in eukaryotic microorganisms”. Annu. Rev. Microbiol. 40: 55-77. PMID 3535653.
- ↑ Hyde, C.C., Ahmed, S.A., Padlan, E.A., Miles, E.W. and Davies, D.R. (1988). „Three-dimensional structure of the tryptophan synthase α2β2 multienzyme complex from Salmonella typhimurium”. J. Biol. Chem. 263: 17857-17871. PMID 3053720.
- ↑ Woehl, E. and Dunn, M.F. (1999). „Mechanisms of monovalent cation action in enzyme catalysis: the tryptophan synthase α-, β-, and αβ-reactions”. Biochemistry 38: 7131-7141. PMID 10353823.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.