Glicin oksidaza
Glicin oksidaza (EC 1.4.3.19, glicinska oksidaza) je enzim sa sistematskim imenom glicin:kiseonik oksidoreduktaza (deaminacija).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju:
| Glicin oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| |||||||||
| Glicin oksidaza tetramer, Geobacillus kaustophilus | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.4.3.19 | ||||||||
| CAS broj | 39307-16-9 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- glicin + H2O + O2 glioksilat + NH3 +H2O2 (sveukupna reakcija)
- (1a) glicin + O2 2-iminoacetat +H2O2
- (1b) 2-iminoacetat +H2O glioksilat + NH3
Ovaj enzim je flavoenzim koji sadrži nekovalentno vezani FAD. Enzim iz Bacillus subtilis je aktivan sa glicinom, sarkozinom, N-etilglicin, D-alanin, D-alfa-aminobutirat, D-prolin, D-pipekolat i Nmetil-D-alanin.
Reference uredi
- ↑ Job, V., Marcone, G.L., Pilone, M.S. and Pollegioni, L. (2002). „Glycine oxidase from Bacillus subtilis. Characterization of a new flavoprotein”. J. Biol. Chem. 277: 6985-6993. PMID 11744710.
- ↑ Nishiya, Y. and Imanaka, T. (1998). „Purification and characterization of a novel glycine oxidase from Bacillus subtilis”. FEBS Lett. 438: 263-266. PMID 9827558.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.