Betain-aldehid dehidrogenaza
Betain-aldehid dehidrogenaza (EC 1.2.1.8, betainska aldehidna oksidaza, BADH, betain aldehidna dehidrogenaza, BetB) je enzim sa sistematskim imenom betain-aldehid:NAD+ oksidoreduktaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Betain-aldehid dehidrogenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| |||||||||
| Betain-aldehid dehidrogenaza tetramer, Staphylococcus aureus | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.2.1.8 | ||||||||
| CAS broj | 9028-90-4 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- betainski aldehid + NAD+ +H2O betain + NADH + 2 H+
Kod mnogih bakterija, biljki i životinja, osmoprotektant betain se sintetiše u dva koraka: (1) holin do betainskog aldehid i (2) betainski aldehid do betain. Ovaj enzim učestvuje u drugom koraku. On se javlja u pojedinim biljkama, životinjama i bakterijama. Različiti enzimi učestvuju u prvoj reakciji. Kod biljki, tu reakciju katalizuje EC 1.14.15.7 (holin monooksigenaza), dok kod životinja i mnogih bakterija nju posreduje bilo za membranu vezana EC 1.1.99.1 (holinska dehidrogenaza) ili rastvorna EC 1.1.3.17 (holinska oksidaza). Kod nekih bakterija, betain se sintetiše iz glicina putem dejstva EC 2.1.1.156 (glycin/sarkosin N-metiltransferaze) i EC 2.1.1.157 (sarkozin/dimetilglicin N-metiltransferaze).
Reference uredi
- ↑ Rothschild, H.A. and Barron, E.S.G. (1954). „The oxidation of betaine aldehyde by betaine aldehyde dehydrogenase”. J. Biol. Chem. 209: 511-523. PMID 13192104.
- ↑ Livingstone, J.R., Maruo, T., Yoshida, I., Tarui, Y., Hirooka, K., Yamamoto, Y., Tsutui, N. and Hirasawa, E. (2003). „Purification and properties of betaine aldehyde dehydrogenase from Avena sativa”. J. Plant Res. 116: 133-140. PMID 12736784.
- ↑ Muñoz-Clares, R.A., González-Segura, L., Mújica-Jiménez, C. and Contreras-Diaz, L. (2003). „Ligand-induced conformational changes of betaine aldehyde dehydrogenase from Pseudomonas aeruginosa and Amaranthus hypochondriacus L. leaves affecting the reactivity of the catalytic thiol”. Chem. Biol. Interact.: 129-137. PMID 12604197.
- ↑ Johansson, K., El-Ahmad, M., Ramaswamy, S., Hjelmqvist, L., Jornvall, H. and Eklund, H. (1998). „Structure of betaine aldehyde dehydrogenase at 2.1 Å resolution”. Protein Sci. 7: 2106-2117. PMID 9792097.
- ↑ Waditee, R., Tanaka, Y., Aoki, K., Hibino, T., Jikuya, H., Takano, J., Takabe, T. and Takabe, T. (2003). „Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica”. J. Biol. Chem. 278: 4932-4942. PMID 12466265.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
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