23S rRNK (adenin2503-C8)-metiltransferaza
23S rRNK (adenin2503-C8)-metiltransferaza (EC 2.1.1.224, Cfr (gen)) je enzim sa sistematskim imenom S-adenozil-L-metionin:23S rRNK (adenin2503-C8)-metiltransferaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 23S rRNK (adenin2503-C8)-metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.224 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 2 S-adenozil-L-metionin + adenin2503 u 23S rRNK S-adenozil-L-homocistein + L-metionin + 5'-dezoksiadenozin + 8-metiladenin2503 u 23S rRNK
Ovaj enzim je član familije AdoMet radikala (radikala SAM). S-adenozil-L-metionin deluje kao generator radikala i kao izvor metil grupa. On sadrži [4Fe-S] klaster. Cfr je plazmidom prenesena metiltransferaza koja štiti ćelije od dejstva antibiotika. Ovaj enzim metiliše adenozin u poziciji 2503 23S rRNK radikalnih mehanizmom. Transfer CH2 grupe sa S-adenozil-L-metionina uz zadržavanje vodonika u C-8 poziciji adenina. On takođe metiliše 2-metiladenin proizveden dejstvom enzima EC 2.1.1.192, 23S rRNK (adenin2503-C2)-metiltransferaze.
Reference uredi
- ↑ Giessing, A.M., Jensen, S.S., Rasmussen, A., Hansen, L.H., Gondela, A., Long, K., Vester, B. and Kirpekar, F. (2009). „Identification of 8-methyladenosine as the modification catalyzed by the radical SAM methyltransferase Cfr that confers antibiotic resistance in bacteria”. RNA 15: 327-336. PMID 19144912.
- ↑ Kaminska, K.H., Purta, E., Hansen, L.H., Bujnicki, J.M., Vester, B. and Long, K.S. (2010). „Insights into the structure, function and evolution of the radical-SAM 23S rRNA methyltransferase Cfr that confers antibiotic resistance in bacteria”. Nucleic Acids Res. 38: 1652-1663. PMID 20007606.
- ↑ Yan, F., LaMarre, J.M., Röhrich, R., Wiesner, J., Jomaa, H., Mankin, A.S., Fujimori, D.G. (2010). „RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA”. J. Am. Chem. Soc. 132: 3953-3964. PMID 20184321.
- ↑ Yan, F. and Fujimori, D.G. (2011). „RNA methylation by Radical SAM enzymes RlmN and Cfr proceeds via methylene transfer and hydride shift”. Proc. Natl. Acad. Sci. USA 108: 3930-3934. PMID 21368151.
- ↑ Grove, T.L., Benner, J.S., Radle, M.I., Ahlum, J.H., Landgraf, B.J., Krebs, C. and Booker, S.J. (2011). „A radically different mechanism for S-adenosylmethionine-dependent methyltransferases”. Science 332: 604-607. PMID 21415317.
- ↑ Boal, A.K., Grove, T.L., McLaughlin, M.I., Yennawar, N.H., Booker, S.J. and Rosenzweig, A.C. (2011). „Structural basis for methyl transfer by a radical SAM enzyme”. Science 332: 544-545. PMID 21527678.
Literatura uredi
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