Di-trans,poli-cis-dekaprenilcistransferaza
(Preusmjereno sa stranice Undekaprenil-difosfat sintaza)
Di-trans,poli-cis-dekaprenilcistransferaza (EC 2.5.1.31, di-trans,poli-cis-undekaprenil-difosfat sintaza, undekaprenil-difosfat sintaza, baktoprenil-difosfatna sintaza, UPP sintetaza, undekaprenil difosfatna sintetaza, undekaprenilna pirofosfat, sintetaza) je enzim sa sistematskim imenom (2E,6E)-farnezil-difosfat:izopentenil-difosfat cistransferaza (dodaje 8 izopentenilnih jedinica).[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Di-trans,poli-cis-dekaprenilcistransferaza | |||||||||
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| Identifikatori | |||||||||
| EC broj | 2.5.1.31 | ||||||||
| CAS broj | 52350-87-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- (2E,6E)-farnezil difosfat + 8 izopentenil difosfat 8 difosfat + ditrans,oktacis-undekaprenil difosfat
Undekaprenil pirofosfatna sintaza katalizuje konsekutivne reakcije kondenzacije farnezil difosfata sa osam izopentenilnih difosfata, u kojima se nove cis-dvostruke veze formiraju. Time se generiše undekaprenil difosfat koji služi kao [[[lipid]]ni nosilac za peptidoglikansku sintezu bakterijskog ćelijskog zida.
Reference uredi
- ↑ Muth, J.D. and Allen, C.M. (1984). „Undecaprenyl pyrophosphate synthetase from Lactobacillus plantarum: a dimeric protein”. Arch. Biochem. Biophys. 230: 49-60. PMID 6712246.
- ↑ Takahashi, I. and Ogura, K. (1982). „Prenyltransferases of Bacillus subtilis: undecaprenyl pyrophosphate synthetase and geranylgeranyl pyrophosphate synthetase”. J. Biochem. (Tokyo) 92: 1527-1537. PMID 6818223.
- ↑ Guo, R.T., Ko, T.P., Chen, A.P., Kuo, C.J., Wang, A.H. and Liang, P.H. (2005). „Crystal structures of undecaprenyl pyrophosphate synthase in complex with magnesium, isopentenyl pyrophosphate, and farnesyl thiopyrophosphate: roles of the metal ion and conserved residues in catalysis”. J. Biol. Chem. 280: 20762-20774. PMID 15788389.
- ↑ Ko, T.P., Chen, Y.K., Robinson, H., Tsai, P.C., Gao, Y.G., Chen, A.P., Wang, A.H. and Liang, P.H. (2001). „Mechanism of product chain length determination and the role of a flexible loop in Escherichia coli undecaprenyl-pyrophosphate synthase catalysis”. J. Biol. Chem. 276: 47474-47482. PMID 11581264.
- ↑ Fujikura, K., Zhang, Y.W., Fujihashi, M., Miki, K. and Koyama, T. (2003). „Mutational analysis of allylic substrate binding site of Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase”. Biochemistry 42: 4035-4041. PMID 12680756.
- ↑ Fujihashi, M., Zhang, Y.W., Higuchi, Y., Li, X.Y., Koyama, T. and Miki, K. (2001). „Crystal structure of cis-prenyl chain elongating enzyme, undecaprenyl diphosphate synthase”. Proc. Natl. Acad. Sci. USA 98: 4337-4342. PMID 11287651.
- ↑ Pan, J.J., Chiou, S.T. and Liang, P.H. (2000). „Product distribution and pre-steady-state kinetic analysis of Escherichia coli undecaprenyl pyrophosphate synthase reaction”. Biochemistry 39: 10936-10942. PMID 10978182.
- ↑ Kharel, Y., Zhang, Y.W., Fujihashi, M., Miki, K. and Koyama, T. (2003). „Significance of highly conserved aromatic residues in Micrococcus luteus B-P 26 undecaprenyl diphosphate synthase”. J. Biochem. 134: 819-826. PMID 14769870.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.