Tiamin oksidaza
Tiamin oksidaza (EC 1.1.3.23, tiaminska dehidrogenaza, tiaminska dehidrogenaza, tiamin:kiseonik 5-oksidoreduktaza) je enzim sa sistematskim imenom tiamin:kiseonik 5-oksidoreduktaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Tiamin oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.3.23 | ||||||||
| CAS broj | 96779-44-1 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- tiamin + 2 O2 +H2O tiamin sirćetna kiselina + 2H2O2
Ovaj enzim je flavoprotein (FAD). Njegov produkt se razlikuje od tiamin po zameni grupe -CH2.CH2.OH grupom -CH2.COOH. Dvostepena oksidacija teče bez otpuštanja intermedijernog aldehida iz enzima.
Reference uredi
- ↑ Edmondson, D.E., Kenney, W.C. and Singer, T.P. (1976). „Structural elucidation and properties of 8α-(N1-histidyl)riboflavin: the flavin component of thiamine dehydrogenase and β-cyclopiazonate oxidocyclase”. Biochemistry 15: 2937-2945. PMID 8076.
- ↑ Gomez-Moreno, C. and Edmondson, D.E. (1985). „Evidence for an aldehyde intermediate in the catalytic mechanism of thiamine oxidase”. Arch. Biochem. Biophys. 239: 46-52. PMID 2988447.
- ↑ Neal, R.A. (1970). „Bacterial metabolism of thiamine. 3. Metabolism of thiamine to 3-(2′-methyl-4′-amino-5′-pyrimidylmethyl)-4-methyl-thiazole-5-acetic acid (thiamine acetic acid) by a flavoprotein isolated from a soil microorganism”. J. Biol. Chem. 245: 2599-2604. PMID 4987737.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.