Taksadienska sintaza
Taksadienska sintaza (EC 4.2.3.17, geranilgeranil-difosfat difosfat-lijaza (ciklizacija, formira taksadien)) je enzim sa sistematskim imenom geranilgeranil-difosfat difosfat-lijaza (ciklizacija, formira taksa-4,11-dien).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Taksadienska sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.3.17 | ||||||||
| CAS broj | 169277-52-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- geranilgeranil difosfat taksa-4,11-dien + difosfat
Ovaj enzim učestvuje u biosintezi diterpenoidnog antineoplastičnog leka Taksol (paklitaksela).
Reference
uredi- ↑ Koepp, A.E., Hezari, M., Zajicek, J., Vogel, B.S., LaFever, R.E., Lewis, N.G. and Croteau, R. (1995). „Cyclization of geranylgeranyl diphosphate to taxa-4(5),11(12)-diene is the committed step of taxol biosynthesis in Pacific yew”. J. Biol. Chem. 270: 8686-8690. PMID 7721772.
- ↑ Hezari, M., Lewis, N.G. and Croteau, R. (1995). „Purification and characterization of taxa-4(5),11(12)-diene synthase from Pacific yew (Taxus brevifolia) that catalyzes the first committed step of taxol biosynthesis”. Arch. Biochem. Biophys. 322: 437-444. PMID 7574719.
- ↑ Lin, X., Hezari, M., Koepp, A.E., Floss, H.G. and Croteau, R. (1996). „Mechanism of taxadiene synthase, a diterpene cyclase that catalyzes the first step of taxol biosynthesis in Pacific yew”. Biochemistry 35: 2968-2977. PMID 8608134.
- ↑ Hezari, M., Ketchum, R.E., Gibson, D.M. and Croteau, R. (1997). „Taxol production and taxadiene synthase activity in Taxus canadensis cell suspension cultures”. Arch. Biochem. Biophys. 337: 185-90. PMID 9016812.
- ↑ Williams, D.C., Carroll, B.J., Jin, Q., Rithner, C.D., Lenger, S.R., Floss, H.G., Coates, R.M., Williams, R.M. and Croteau, R. (2000). „Intramolecular proton transfer in the cyclization of geranylgeranyl diphosphate to the taxadiene precursor of taxol catalyzed by recombinant taxadiene synthase”. Chem. Biol. 7: 969-977. PMID 11137819.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.