Retinol dehidrogenaza
Retinol dehidrogenaza (EC 1.1.1.105, retinol (vitamin A1) dehidrogenaza, MDR, mikrozomalna retinolna dehidrogenaza, retinalna reduktaza, retinenska reduktaza, epidermalna retinol dehidrogenaza 2, SDR16C5, RDH16) je enzim sa sistematskim imenom all-trans retinol:NAD+ oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Retinol dehidrogenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.105 | ||||||||
| CAS broj | 9033-53-8 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- sve-trans-retinol---[ćelijski protein vezivanja retinola] + NAD+ sve-trans-retinal---[ćelijski protein vezivanja retinola] + NADH + H+
Retinolna dehidrogenaza prepoznaje sve-trans-retinol i sve-trans-retinal kao supstrate i ispoljava jaku preferenciju za NAD+/NADH kao kofaktore. Ona prepoznaje supstrat u slobodnoj formi, kao i kad je vezan za ćelijski-retinol-vezujući-protein (CRBP1), ali ima veći afinitet za supstrat u vezanoj formi. Retinolna dehidrogenaza nije aktivna na 11-cis-retinolu ili 11-cis-retinala (cf. EC 1.1.1.315, 11-cis retinol dehidrogenaza). Ona je deluje na 3alfa-hidroksisteroide.
Reference uredi
- ↑ Koen, A.L. and Shaw, C.R. (1966). „Retinol and alcohol dehydrogenases in retina and liver”. Biochim. Biophys. Acta 128: 48-54. PMID 5972368.
- ↑ Gough, W.H., VanOoteghem, S., Sint, T. and Kedishvili, N.Y. (1998). „cDNA cloning and characterization of a new human microsomal NAD+-dependent dehydrogenase that oxidizes all-trans-retinol and 3α-hydroxysteroids”. J. Biol. Chem. 273: 19778-19785. PMID 9677409.
- ↑ Matsuzaka, Y., Okamoto, K., Tsuji, H., Mabuchi, T., Ozawa, A., Tamiya, G. and Inoko, H. (2002). „Identification of the hRDH-E2 gene, a novel member of the SDR family, and its increased expression in psoriatic lesion”. Biochem. Biophys. Res. Commun. 297: 1171-1180. PMID 12372410.
- ↑ Lee, S.A., Belyaeva, O.V. and Kedishvili, N.Y. (2009). „Biochemical characterization of human epidermal retinol dehydrogenase 2”. Chem. Biol. Interact. 178: 182-187. PMID 18926804.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.