Rhizobium leguminosarum eksopolisaharid glukozil ketal-piruvat-transferaza
Rhizobium leguminosarum eksopolisaharid glukozil ketal-piruvat-transferaza (EC 2.5.1.98, PssM) je enzim sa sistematskim imenom fosfoenolpiruvat:(D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-(3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6))-2(or3)-O-Ac-D-Glc-alfa-(1->6))n 4,6-O-(1-karboksiethan-1,1-diil)transferaza.[1] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Rhizobium leguminosarum eksopolisaharid glukozil ketal-piruvat-transferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.5.1.98 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- fosfoenolpiruvat + [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(ili 3)-O-Ac-D-Glc-alfa-(1->6)]n [D-GlcA-beta-(1->4)-2-O-Ac-D-GlcA-beta-(1->4)-D-Glc-beta-(1->4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal--beta-(1->3)-4,6-CH3(COO-)C-D-Glc-beta-(1->4)-D-Glc-beta-(1->4)-D-Glc-beta-(1->6)]-2(ili 3)-O-Ac-D-Glc-alfa-(1->6)]n + fosfat
Ovaj enzim je odgovoran za piruvilaciju subterminalne glukoze u kiseloj oktasaharidnoj ponavljajućoj jedinici eksopolisaharida kod Rhizobium leguminosarum (bv. viciae soj VF39), koja je neophodna za uspostavlanje azot-fiksirajuće simbioze sa Pisum sativum, Vicia faba, i Vicia sativa.
Reference uredi
- ↑ Ivashina, T.V., Fedorova, E.E., Ashina, N.P., Kalinchuk, N.A., Druzhinina, T.N., Shashkov, A.S., Shibaev, V.N. and Ksenzenko, V.N. (2010). „Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae—Pisum sativum symbiosis”. J. Appl. Microbiol. 109: 731-742. PMID 20233262.
Literatura uredi
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