RRNK mala podjedinica pseudouridinske metiltransferaze Nep1
(Preusmjereno sa stranice RRNK mala podjedinica pseudouridinske metiltransferaze (Nep1))
rRNK mala podjedinica pseudouridinske metiltransferaze Nep1 (EC 2.1.1.260, Nep1, nukleolarni esencijalni protein 1) je enzim sa sistematskim imenom S-adenozil-L-metionin:18S rRNK (pseudouridin1191-N1)-metiltransferaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
rRNK mala podjedinica pseudouridinske metiltransferaze Nep1 | |||||||||
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Identifikatori | |||||||||
EC broj | 2.1.1.260 | ||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- S-adenozil-L-metionin + pseudouridin1191 u kvašćanoj 18S rRNK S-adenozil-L-homocistein + N1-metilpseudouridin1191 u kvašćanoj 18S rRNK
Ovaj enzim se javlja kod prokariota i eukariota. On specifično prepoznaje pseudouridinske ostatke (Psi) u malim podjedinicama ribozomalne RNK na bazi lokalne RNK strukture.
Reference uredi
- ↑ Taylor, A.B., Meyer, B., Leal, B.Z., Kötter, P., Schirf, V., Demeler, B., Hart, P.J., Entian, K.-D. and Wöhnert, J. (2008). „The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site”. Nucleic Acids Res. 36: 1542-1554. PMID 18208838.
- ↑ Wurm, J.P., Meyer, B., Bahr, U., Held, M., Frolow, O., Kötter, P., Engels, J.W., Heckel, A., Karas, M., Entian, K.-D. and Wöhnert, J. (2010). „The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase”. Nucleic Acids Res. 38: 2387-2398. PMID 20047967.
- ↑ Meyer, B., Wurm, J.P., Kötter, P., Leisegang, M.S., Schilling, V., Buchhaupt, M., Held, M., Bahr, U., Karas, M., Heckel, A., Bohnsack, M.T., Wöhnert, J. and Entian, K.-D. (2011). „The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA”. Nucleic Acids Res. 39: 1526-1537. PMID 20972225.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
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