PreQ1 sintaza
PreQ1 sintaza (EC 1.7.1.13, YkvM, QueF, preQ0 reduktaza, preQ0 oksidoreduktaza, 7-cijano-7-deazaguaninska reduktaza) je enzim sa sistematskim imenom 7-aminometil-7-karbaguanin:NADP+ oksidoreduktaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| PreQ1 sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.7.1.13 | ||||||||
| CAS broj | 1256460-80-6 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 7-aminometil-7-karbaguanin + 2 NADP+ 7-cijano-7-karbaguanin + 2 NADPH + 2 H+
Reakcija se odvija u revernom smeru. Ovaj enzim katalizuje jedan od ranih koraka sinteze kjuozina (Q-tRNK). Njemu sledi reakcija posredovana enzimom EC 2.4.2.29, kjuozin tRNK-riboziltransferaze.
Reference
uredi- ↑ Van Lanen, S.G., Reader, J.S., Swairjo, M.A., de Crécy-Lagard, V., Lee, B. and Iwata-Reuyl, D. (2005). „From cyclohydrolase to oxidoreductase: discovery of nitrile reductase activity in a common fold”. Proc. Natl. Acad. Sci. USA 102: 4264-4269. PMID 15767583.
- ↑ Yokoyama, S., Miyazawa, T., Iitaka, Y., Yamaizumi, Z., Kasai, H. and Nishimura, S. (1979). „Three-dimensional structure of hyper-modified nucleoside Q located in the wobbling position of tRNA”. Nature 282: 107-109. PMID 388227.
- ↑ Kuchino, Y., Kasai, H., Nihei, K. and Nishimura, S. (1976). „Biosynthesis of the modified nucleoside Q in transfer RNA”. Nucleic Acids Res. 3: 393-398. PMID 1257053.
- ↑ Okada, N., Noguchi, S., Nishimura, S., Ohgi, T., Goto, T., Crain, P.F. and McCloskey, J.A. (1978). „Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine”. Nucleic Acids Res. 5: 2289-2296. PMID 353740.
- ↑ Noguchi, S., Yamaizumi, Z., Ohgi, T., Goto, T., Nishimura, Y., Hirota, Y. and Nishimura, S. (1978). „Isolation of Q nucleoside precursor present in tRNA of an E. coli mutant and its characterization as 7-(cyano)-7-deazaguanosine”. Nucleic Acids Res. 5: 4215-4223. PMID 364423.
- ↑ Swairjo, M.A., Reddy, R.R., Lee, B., Van Lanen, S.G., Brown, S., de Crécy-Lagard, V., Iwata-Reuyl, D. and Schimmel, P. (2005). „Crystallization and preliminary X-ray characterization of the nitrile reductase QueF: a queuosine-biosynthesis enzyme”. Acta Crystallogr. F Struct. Biol. Cryst. Commun. 61: 945-948. PMID 16511203.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.