Pentahlorofenol monooksigenaza
(Preusmjereno sa stranice PCB 4-monooxygenase)
Pentahlorofenol monooksigenaza (EC 1.14.13.50, pentahlorofenolna dehlorinase, pentahlorofenolna dehalogenaza, pentahlorofenolna 4-monooksigenaza, PCP hidroksilaza, pentahlorofenolna hidroksilaza, PcpB, PCB 4-monooksigenaza, PCB4MO) je enzim sa sistematskim imenom pentahlorofenol,NADPH:kiseonik oksidoreduktaza (hidroksilacija, dehlorinacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Pentahlorofenol monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.50 | ||||||||
| CAS broj | 136111-57-4 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) pentahlorofenol + 2 NADPH + H+ + O2 2,3,5,6-tetrahlorohidrohinon + 2 NADP+ + hlorid +H2O
- (2) 2,3,5,6-tetrahlorofenol + NADPH + H+ + O2 2,3,5,6-tetrahlorohidrohinon + NADP+ +H2O
Ovaj enzim je flavoprotein (FAD).
Reference uredi
- ↑ Schenk, T., Müller, R., Mörsberger, F., Otto, M.K. and Lingens, F. (1989). „Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790”. J. Bacteriol. 171: 5487-5491. PMID 2793827.
- ↑ Xun, L., Topp, E. and Orser, C.S. (1992). „Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries”. J. Bacteriol. 174: 2898-2902. PMID 1569020.
- ↑ Xun, L., Topp, E. and Orser, C.S. (1992). „Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase”. J. Bacteriol. 174: 5745-5747. PMID 1512208.
- ↑ Lange, C.C., Schneider, B.J. and Orser, C.S. (1996). „Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723”. Biochem. Biophys. Res. Commun. 219: 146-149. PMID 8619798.
- ↑ Nakamura, T., Motoyama, T., Hirono, S. and Yamaguchi, I. (2004). „Identification, characterization, and site-directed mutagenesis of recombinant pentachlorophenol 4-monooxygenase”. Biochim. Biophys. Acta 1700: 151-159. PMID 15262224.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.