HtrA2 peptidaza
(Preusmjereno sa stranice OMI/HtrA2 protease)
HtrA2 peptidaza (EC 3.4.21.108, protein A2 sa zahtevom za visoku temperaturu, HtrA2, Omi stres-regulišuća endoproteaza, serinska proteinaza OMI, HtrA2 proteaza, OMI/HtrA2 proteaza, HtrA2/Omi, Omi/HtrA2) je enzim.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| HtrA2 peptidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.21.108 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- Odvajanje nepolarne alifatične aminokiseline na P1 poziciji, sa preferencijom za Val, Ile i Met. U P2 i P3 poziciji je najčešće Arg sa sekundarnom preferencijom za druge hidrofilne ostatake
Izražavanje ovog enzima je povišeno u sisarskim ćelijama u odgovoru na stres indukovan toplotnim šokom i tunikamicinskim tretmanom, a ima značaj i u patogenezi Parkinsonove bolesti.
Reference
uredi- ↑ Srinivasula, S.M., Gupta, S., Datta, P., Zhang, Z., Hegde, R., Cheong, N., Fernandes-Alnemri, T. and Alnemri, E.S. (2003). „Inhibitor of apoptosis proteins are substrates for the mitochondrial serine protease Omi/HtrA2”. J. Biol. Chem. 278: 31469-31472. PMID 12835328.
- ↑ Savopoulos, J.W., Carter, P.S., Turconi, S., Pettman, G.R., Karran, E.H., Gray, C.W., Ward, R.V., Jenkins, O. and Creasy, C.L. (2000). „Expression, purification, and functional analysis of the human serine protease HtrA2”. Protein. Expr. Purif. 19: 227-234. PMID 10873535.
- ↑ Martins, L.M., Turk, B.E., Cowling, V., Borg, A., Jarrell, E.T., Cantley, L.C. and Downward, J. (2003). „Binding specificity and regulation of the serine protease and PDZ domains of HtrA2/Omi”. J. Biol. Chem. 278: 49417-49427. PMID 14512424.
- ↑ Gray, C.W., Ward, R.V., Karran, E., Turconi, S., Rowles, A., Viglienghi, D., Southan, C., Barton, A., Fantom, K.G., West, A., Savopoulos, J., Hassan, N.J., Clinkenbeard, H., Hanning, C., Amegadzie, B., Davis, J.B., Dingwall, C., Livi, G.P. and Creasy, C.L. (2000). „Characterization of human HtrA2, a novel serine protease involved in the mammalian cellular stress response”. Eur. J. Biochem. 267: 5699-5710. PMID 10971580.
- ↑ Li, W., Srinivasula, S.M., Chai, J., Li, P., Wu, J.W., Zhang, Z., Alnemri, E.S. and Shi, Y. (2002). „Structural insights into the pro-apoptotic function of mitochondrial serine protease HtrA2/Omi”. Nat. Struct. Biol. 9: 436-441. PMID 11967569.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.