N-acetilglukozaminil-difosfo-dekaprenol L-ramnoziltransferaza
N-acetilglukozaminil-difosfo-dekaprenol L-ramnoziltransferaza (EC 2.4.1.289, WbbL) je enzim sa sistematskim imenom dTDP-6-dezoksi-beta-L-manoza:N-acetil-alfa-D-glukozaminil-difosfo-trans,oktacis-dekaprenol 3-alfa-L-ramnoziltransferaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| N-acetilglukozaminil-difosfo-dekaprenol L-ramnoziltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.4.1.289 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- dTDP-6-dezoksi-beta-L-manoza + N-acetil-alfa-D-glukozaminil-difosfo-trans,oktacis-dekaprenol dTDP + alfa-L-ramnopiranozil-(1->3)-N-acetil-alfa-D-glukozaminil-difosfo-trans,oktacis-dekaprenol
Za dejstvo ovog enzima je neophodan Mn2+ ili Mg2+. On je izolovan iz Mycobakterija smegmatis i Mycobakterija tuberculosis.
Reference uredi
- ↑ Mills, J.A., Motichka, K., Jucker, M., Wu, H.P., Uhlik, B.C., Stern, R.J., Scherman, M.S., Vissa, V.D., Pan, F., Kundu, M., Ma, Y.F. and McNeil, M. (2004). „Inactivation of the mycobacterial rhamnosyltransferase, which is needed for the formation of the arabinogalactan-peptidoglycan linker, leads to irreversible loss of viability”. J. Biol. Chem. 279: 43540-43546. PMID 15294902.
- ↑ Grzegorzewicz, A.E., Ma, Y., Jones, V., Crick, D., Liav, A. and McNeil, M.R. (2008). „Development of a microtitre plate-based assay for lipid-linked glycosyltransferase products using the mycobacterial cell wall rhamnosyltransferase WbbL”. Microbiology 154: 3724-3730. PMID 19047740.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.