Metilmalonat-semialdehid dehidrogenaza (acilacija)
(Preusmjereno sa stranice MMSA dehidrogenaza)
Metilmalonat-semialdehid dehidrogenaza (acilacija) (EC 1.2.1.27, MSDH, MMSA dehidrogenaza) je enzim sa sistematskim imenom 2-metil-3-oksopropanoat:NAD+ 3-oksidoreduktaza (KoA-propanoilacija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Metilmalonat-semialdehid dehidrogenaza (acilacija) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
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| Tetramer metilmalonat-semialdehid dehidrogenaze (Bacillus subtilis) | |||||||||
| Identifikatori | |||||||||
| EC broj | 1.2.1.27 | ||||||||
| CAS broj | 37205-49-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- 2-metil-3-oksopropanoat + KoA +H2O + NAD+ propanoil-KoA + HCO3- + NADH
Ovaj enzim takođe konvertuje 3-oksopropanoat u acetil-KoA. Reakcija se odvija u dva koraka, pri čemu dekarboksilacioni process teče uz KoA vezivanje. Bikarbonat umesto CO2 se oslobađa kao finalni produkt.
Reference uredi
- ↑ Sokatch, J.R., Sanders, L.E. and Marshall, V.P. (1968). „Oxidation of methylmalonate semialdehyde to propionyl coenzyme A in Pseudomonas aeruginosa grown on valine”. J. Biol. Chem. 243: 2500-2506. PMID 4297649.
- ↑ Dubourg, H., Stines-Chaumeil, C., Didierjean, C., Talfournier, F., Rahuel-Clermont, S., Branlant, G. and Aubry, A. (2004). „Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis”. Acta Crystallogr. D Biol. Crystallogr. 60: 1435-1437. PMID 15272169.
- ↑ Stines-Chaumeil, C., Talfournier, F. and Branlant, G. (2006). „Mechanistic characterization of the MSDH (methylmalonate semialdehyde dehydrogenase) from Bacillus subtilis”. Biochem. J. 395: 107-115. PMID 16332250.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.