M7GpppX difosfataza
(Preusmjereno sa stranice M7GpppX pyrophosphatase)
M7GpppX difosfataza (EC 3.6.1.59, DcpS, m7GpppX pirofosfataza, m7GpppN m7GMP fosfohidrolaza) je enzim sa sistematskim imenom m7G5'ppp5'N m7GMP fosfohidrolaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| M7GpppX difosfataza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.6.1.59 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) m7G5'ppp5'N(3'ppp5'N)n + H2O 7-metilguanozin 5'-fosfat + pp5'N(3'ppp5'N)n
- (2) 7-metilguanozin 5'-difosfat + H2O 7-metilguanozin 5'-fosfat + fosfat
Ovaj enzim posreduje uklanjanje kape i import tokom eukariotske iRNK degradacije.
Reference
uredi- ↑ Malys, N. and McCarthy, J.E. (2006). „Dcs2, a novel stress-induced modulator of m7GpppX pyrophosphatase activity that locates to P bodies”. J. Mol. Biol. 363: 370-382. PMID 16963086.
- ↑ Liu, S.W., Rajagopal, V., Patel, S.S. and Kiledjian, M. (2008). „Mechanistic and kinetic analysis of the DcpS scavenger decapping enzyme”. J. Biol. Chem. 283: 16427-16436. PMID 18441014.
- ↑ Liu, H., Rodgers, N.D., Jiao, X. and Kiledjian, M. (2002). „The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases”. EMBO J. 21: 4699-4708. PMID 12198172.
- ↑ van Dijk, E., Le Hir, H. and Seraphin, B. (2003). „DcpS can act in the 5′-3′ mRNA decay pathway in addition to the 3′-5′ pathway”. Proc. Natl. Acad. Sci. USA 100: 12081-12086. PMID 14523240.
- ↑ Chen, N., Walsh, M.A., Liu, Y., Parker, R. and Song, H. (2005). „Crystal structures of human DcpS in ligand-free and m7GDP-bound forms suggest a dynamic mechanism for scavenger mRNA decapping”. J. Mol. Biol. 347: 707-718. PMID 15769464.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.