Lizin 6-dehidrogenaza
Lizin 6-dehidrogenaza (EC 1.4.1.18, L-lizin epsilon-dehidrogenaza, L-lizin 6-dehidrogenaza, LysDH) je enzim sa sistematskim imenom L-lizin:NAD+ 6-oksidoreduktaza (deaminacija).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Lizin 6-dehidrogenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.4.1.18 | ||||||||
| CAS broj | 89400-30-6 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- L-lizin + NAD+ (S)-2,3,4,5-tetrahidropiridin-2-karboksilat + NADH + H+ + NH3 (sveukupna reakcija)
- (1a) L-lizin + NAD+ +H2O (S)-2-amino-6-oksoheksanoat + NADH + H+ + NH3
- (1b) (S)-2-amino-6-oksoheksanoat (S)-2,3,4,5-tetrahidropiridin-2-karboksilat +H2O (spontana reakcija)
Ovaj enzim je visoko specifična za L-lizin kao supstrat. On takođe može da koristi S-(2-aminoetil)-L-cistein, mada sporije.
Reference
uredi- ↑ Misono, H. and Nagasaki, S. (1982). „Occurrence of L-lysine ε-dehydrogenase in Agrobacterium tumefaciens”. J. Bacteriol. 150: 398-401. PMID 6801024.
- ↑ Misono, H., Uehigashi, H., Morimoto, E. and Nagasaki, S. (1985). „Purification and properties of L-lysine ε-dehydrogenase from Agrobacterium tumefaciens”. Agric. Biol. Chem. 49: 2253-2255.
- ↑ Misono, H., Hashimoto, H., Uehigashi, H., Nagata, S. and Nagasaki, S. (1989). „Properties of L-lysine ε-dehydrogenase from Agrobacterium tumefaciens”. J. Biochem. (Tokyo) 105: 1002-1008. PMID 2768207.
- ↑ Heydari, M., Ohshima, T., Nunoura-Kominato, N. and Sakuraba, H. (2004). „Highly stable L-lysine 6-dehydrogenase from the thermophile Geobacillus stearothermophilus isolated from a Japanese hot spring: characterization, gene cloning and sequencing, and expression”. Appl. Environ. Microbiol. 70: 937-942. PMID 14766574.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.