3-ketosteroid 9alfa-monooksigenaza
(Preusmjereno sa stranice KshAB)
3-ketosteroid 9alfa-monooksigenaza (EC 1.14.13.142, KshAB, 3-ketosteroid 9alfa-hidroksilaza) je enzim sa sistematskim imenom androsta-1,4-dien-3,17-dion,NADH:kiseonik oksidoreduktaza (9alfa-hidroksilacija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 3-ketosteroid 9alfa-monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.142 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- androsta-1,4-dien-3,17-dion + NADH + H+ + O2 9alfa-hidroksiandrosta-1,4-dien-3,17-dion + NAD+ + H2O
Ovaj enzim učestvuje u holesterol degradaciji u nekoliko bakterijskih patogena, kao što je Mycobakterija tuberculosis.
Reference uredi
- ↑ Petrusma, M., Dijkhuizen, L. and van der Geize, R. (2009). „Rhodococcus rhodochrous DSM 43269 3-ketosteroid 9α-hydroxylase, a two-component iron-sulfur-containing monooxygenase with subtle steroid substrate specificity”. Appl. Environ. Microbiol. 75: 5300-5307. PMID 19561185.
- ↑ Capyk, J.K., D'Angelo, I., Strynadka, N.C. and Eltis, L.D. (2009). „Characterization of 3-ketosteroid 9α-hydroxylase, a Rieske oxygenase in the cholesterol degradation pathway of Mycobacterium tuberculosis”. J. Biol. Chem. 284: 9937-9946. PMID 19234303.
- ↑ Capyk, J.K., Casabon, I., Gruninger, R., Strynadka, N.C. and Eltis, L.D. (2011). „Activity of 3-ketosteroid 9α-hydroxylase (KshAB) indicates cholesterol side chain and ring degradation occur simultaneously in Mycobacterium tuberculosis”. J. Biol. Chem.: -. PMID 21987574.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.