Kostunolid sintaza
Kostunolid sintaza (EC 1.14.13.120, kostunolidna sintaza) je enzim sa sistematskim imenom germakra-1(10),4,11(13)-trien-12-oat,NADPH:kiseonik oksidoreduktaza (6alfa-hidroksilacija).[1] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Kostunolid sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.120 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- germakra-1(10),4,11(13)-trien-12-oat + NADPH + H+ + O2 (+)-kostunolid + NADP+ + 2H2O
A heme-tiolate protein (P-450). Ovaj enzim hidroksiliše ugljenik C-6 germakra-1(10),4,11(13)-trien-12-oata i daje 6alfa-hidroksigermakra-1(10),4,11(13)-trien-12-oat, koji se spontano ciklizuje i formira laktonski prsten.
Reference uredi
- ↑ de Kraker, J.W., Franssen, M.C., Joerink, M., de Groot, A. and Bouwmeester, H.J. (2002). „Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demonstration of cytochrome p450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory”. Plant Physiol. 129: 257-268. PMID 12011356.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.