Karboksipeptidaza D
Karboksipeptidaza D (EC 3.4.16.6, serinska karboksipeptidaza II žitarica, Saccharomyces cerevisiae KEX1 genski produkt, karboksipeptidaza Kex1, gen KEX1 serinska karboksipeptidaza, KEX1 karboksipeptidaza, KEX1 proteinaza, KEX1DELTAp, CPDW-II, serinska karboksipeptidaza, Phaseolus proteinaza) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Karboksipeptidaza D | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.16.6 | ||||||||
| CAS broj | 153967-26-1 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- Preferenciono odvajanje C-terminalnih argininskih ili lizinskih ostataka
Ova karboksipeptidaza je optimalno aktivan na pH 4.5-6.0. Inhibira je diizopropil fluorofosfat, i senzitivna je na tiol-blokirajuće reagense.
Reference
uredi- ↑ Breddam, K. (1986). „Serine carboxypeptidases. A review”. Carlsberg Res. Commun. 51: 83-128.
- ↑ Breddam, K., Sørensen, S.B. and Svendsen, I. (1987). „Primary structure and enzymatic properties of carboxypeptidase II from wheat bran”. Carlsberg Res. Commun. 52: 297-311.
- ↑ Dmochowska, A., Dignard, D., Henning, D., Thomas, D.Y. and Bussey, H. (1987). „Yeast KEX1 gene encodes a putative protease with a carboxypeptidase B-like function involved in killer toxin and α-factor precursor processing”. Cell 50: 573-584. PMID 3301004.
- ↑ Liao, D.-I., Breddam, K., Sweet, R.M., Bullock, T. and Remington, S.J. (1992). „Refined atomic model of wheat serine carboxypeptidase II at 2.2-Å resolution”. Biochemistry 31: 9796-9812. PMID 1390755.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.