Izoflavonoid sintaza
Izoflavonoid sintaza (EC 1.14.13.136, CYT93C, IFS, 2-hidroksiizoflavanonska sintaza (nespecifična)) je enzim sa sistematskim imenom likviritigenin,NADPH:kiseonik oksidoreduktaza (hidroksilacija, aril migracija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Izoflavonoid sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.136 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- liquiritigenin + O2 + NADPH + H+ 2,7,4'-trihidroksiizoflavanon + H2O + NADP+
Za rad ovog enzim je neophodan citohrom P450. Reakcija obuhvata migraciju 2-fenil grupe flavanon likviritigenina do 3-pozicije izoflavanona.
Reference uredi
- ↑ Hashim, M.F., Hakamatsuka, T., Ebizuka, Y. and Sankawa, U. (1990). „Reaction mechanism of oxidative rearrangement of flavanone in isoflavone biosynthesis”. FEBS Lett. 271: 219-222. PMID 2226805.
- ↑ Sawada, Y., Kinoshita, K., Akashi, T., Aoki, T. and Ayabe, S. (2002). „Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase”. Plant J. 31: 555-564. PMID 12207646.
- ↑ Sawada, Y. and Ayabe, S. (2005). „Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue”. Biochem. Biophys. Res. Commun. 330: 907-913. PMID 15809082.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.