Hondroitinska B lijaza
Hondroitinska B lijaza (EC 4.2.2.19, hondroitinaza B, ChonB, ChnB) je enzim sa sistematskim imenom hondroitin B lijaza.[1][2][3][4][5][6][7][8][9][10] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Hondroitinska B lijaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.2.19 | ||||||||
| CAS broj | 52227-83-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- Eliminativno razlaganje dermatan sulfata koji sadrži (1->4)-beta-D-heksozaminil i (1->3)-beta-D-glukurozonil ili (1->3)-alfa-L-iduronozil veze do disaharida koji sadrže 4-dezoksi-beta-D-gluk-4-enuronozil grupe čime se formira 4,5-nezasićeni dermatan-sulfatni disaharid (DeltaUA-GalNAc-4S).
Ovaj enzim je jedina lijaza koja je specifična za dermatan sulfat kao supstrat.
Reference uredi
- ↑ Gu, K., Linhardt, R.J., Laliberte, M., Gu, K. and Zimmermann, J. (1995). „Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum”. Biochem. J. 312: 569-577. PMID 8526872.
- ↑ Pojasek, K., Raman, R., Kiley, P., Venkataraman, G. and Sasisekharan, R. (2000). „Biochemical characterization of the chondroitinase B active site”. J. Biol. Chem. 277: 31179-31186. PMID 12063249.
- ↑ Pojasek, K., Shriver, Z., Kiley, P., Venkataraman, G. and Sasisekharan, R. (2001). „Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum”. Biochem. Biophys. Res. Commun. 286: 343-351. PMID 11500043.
- ↑ Suzuki, K., Terasaki, Y. and Uyeda, M. (2002). „Inhibition of hyaluronidases and chondroitinases by fatty acids. J”. Enzyme 17: 183-186. PMID 12443044.
- ↑ Ototani, N. and Yosizawa, Z. (1979). „Purification of chondroitinase B and chondroitinase C using glycosaminoglycan-bound AH-Sepharose 4B”. Carbohydr. Res. 70: 295-306. PMID 427837.
- ↑ Tkalec, A.L., Fink, D., Blain, F., Zhang-Sun, G., Laliberte, M., Bennett, D.C., Gu, K., Zimmermann, J.J. and Su, H. (2000). „Isolation and expression in Escherichia coli of cslA and cslB, genes coding for the chondroitin sulfate-degrading enzymes chondroitinase AC and chondroitinase B, respectively, from Flavobacterium heparinum”. Appl. Environ. Microbiol. 66: 29-35. PMID 10618199.
- ↑ Michel, G., Pojasek, K., Li, Y., Sulea, T., Linhardt, R.J., Raman, R., Prabhakar, V., Sasisekharan, R. and Cygler, M. (2004). „The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides unravels a calcium-dependent catalytic machinery”. J. Biol. Chem. 279: 32882-32896. PMID 15155751.
- ↑ Li, Y., Matte, A., Su, H. and Cygler, M. (1999). „Crystallization and preliminary X-ray analysis of chondroitinase B from Flavobacterium heparinum”. Acta Crystallogr. D Biol. Crystallogr. 55: 1055-1057. PMID 10216304.
- ↑ Huang, W., Matte, A., Li, Y., Kim, Y.S., Linhardt, R.J., Su, H. and Cygler, M. (1999). „Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 Å resolution”. J. Mol. Biol. 294: 1257-1269. PMID 10600383.
- ↑ Huckerby, T.N., Nieduszynski, I.A., Giannopoulos, M., Weeks, S.D., Sadler, I.H. and Lauder, R.M. (2005). „Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides”. FEBS J. 272: 6276-6286. PMID 16336265.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.