Homospermidin sintaza
Homospermidin sintaza (EC 2.5.1.44, Homospermidine synthase) je enzim sa sistematskim imenom putrescin:putrescin 4-aminobutiltransferaza (formira amonijak).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Homospermidin sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.5.1.44 | ||||||||
| CAS broj | 76106-84-8 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) 2 putrescin sim-homospermidin + NH3 + H+
- (2) putrescin + spermidin sim-homospermidin + propan-1,3-diamin
Reakcija ovog enzima se odvija u tri koraka, pri čemu su neki od intermedijera vezani za enzim: NAD+-zavisna dehidrogenacija putrescina, transfer 4-aminobutilidenske grupe sa dehidroputrescina na drugi molekul putrescina i redukcija iminskog intermedijera čime se formira homospermidin.
Reference uredi
- ↑ Tait, G.H. (1979). „The formation of homospermidine by an enzyme from Rhodopseudomonas viridis”. Biochem. Soc. Trans. 7: 199-200. PMID 437275.
- ↑ Böttcher, F., Ober, D. and Hartmann, T. (1994). „Biosynthesis of pyrrolizidine alkaloids: putrescine and spermidine are essential substrates of enzymatic homospermidine formation”. Can. J. Chem. 72: 80-85.
- ↑ Yamamoto, S., Nagata, S. and Kusaba, K. (1993). „Purification and characterization of homospermidine synthase in Acinetobacter tartarogens ATCC 31105”. J. Biochem. 114: 45-49. PMID 8407874.
- ↑ Srivenugopal, K.S. and Adiga, P.R. (1980). „Enzymatic synthesis of sym-homospermidine in Lathyrus sativus T (grass pea) seedlings”. Biochem. J. 190: 461-464. PMID 7470060.
- ↑ Ober, D., Tholl, D., Martin, W. and Hartmann, T. (1996). „Homospermidine synthase of Rhodopseudomonas viridis: Substrate specificity and effects of the heterologously expressed enzyme on polyamine metabolism of Escherichia coli”. J. Gen. Appl. Microbiol. 42: 411-419.
- ↑ Ober, D. and Hartmann, T. (1999). „Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase”. Proc. Natl. Acad. Sci. USA 96: 14777-14782. PMID 10611289.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.