Homocitrat sintaza
Homocitrat sintaza (EC 2.3.3.14, 2-hidroksibutan-1,2,4-trikarboksilat 2-oksoglutarat-lijaza (KoA-acetilacija), acetil-koenzim A:2-ketoglutarat C-acetil transferaza, homocitrat sintetaza, HCS) je enzim sa sistematskim imenom acetil-KoA:2-oksoglutarat C-acetiltransferaza (tioestarska hidroliza, formiranje karboksimetila).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Homocitrat sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| |||||||||
| Homocitrat sintaza homodimer, Schizosaccharomyces pombe | |||||||||
| Identifikatori | |||||||||
| EC broj | 2.3.3.14 | ||||||||
| CAS broj | 9075-60-9 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- acetil-KoA + H2O + 2-oksoglutarat (2R)-2-hidroksibutan-1,2,4-trikarboksilat + KoA
Ovaj enzim učestvuje u alfa-aminoadipatnom putu sinteze lizina, zajedno sa EC 4.2.1.36, homoakonitatnom hidratazom.
Reference uredi
- ↑ Strassman, M. and Ceci, L.N. (1964). „Enzymatic formation of homocitric acid, an intermediate in lysine biosynthesis”. Biochem. Biophys. Res. Commun. 14: 262-267. PMID 5836514.
- ↑ Wulandari, A.P., Miyazaki, J., Kobashi, N., Nishiyama, M., Hoshino, T. and Yamane, H. (2002). „Characterization of bacterial homocitrate synthase involved in lysine biosynthesis”. FEBS Lett. 522: 35-40. PMID 12095615.
- ↑ Andi, B., West, A.H. and Cook, P.F. (2004). „Kinetic mechanism of histidine-tagged homocitrate synthase from Saccharomyces cerevisiae”. Biochemistry 43: 11790-11795. PMID 15362863.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.