Holesterol 25-hidroksilaza
Holesterol 25-hidroksilaza (EC 1.14.99.38, holesterolna 25-monooksigenaza) je enzim sa sistematskim imenom holesterol,vodonik-donor:kiseonik oksidoreduktaza (25-hidroksilacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju:
| Holesterol 25-hidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.99.38 | ||||||||
| CAS broj | 60202-07-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- holesterol + AH2 + O2 25-hidroksiholesterol + A + H2O
Za razliku od većine drugih sterolnih hidroksilaza, ovaj enzim nije citohrom P-450. On koristi kofaktore sa dva atoma gvožđa da katalizuje hidroksilaciju hidrofobnih supstrata.
Reference uredi
- ↑ Lund, E.G., Kerr, T.A., Sakai, J., Li, W.P. and Russell, D.W. (1998). „cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism”. J. Biol. Chem. 273: 34316-34327. PMID 9852097.
- ↑ Chen, J.J., Lukyanenko, Y. and Hutson, J.C. (2002). „25-Hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro”. Biol. Reprod. 66: 1336-1341. PMID 11967195.
- ↑ Lukyanenko, Y., Chen, J.J. and Hutson, J.C. (2002). „Testosterone regulates 25-hydroxycholesterol production in testicular macrophages”. Biol. Reprod. 67: 1435-1438. PMID 12390873.
- ↑ Fox, B.G., Shanklin, J., Ai, J., Loehr, T.M. and Sanders-Loehr, J. (1994). „Resonance Raman evidence for an Fe-O-Fe center in stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins”. Biochemistry 33: 12776-12786. PMID 7947683.
- ↑ Russell, D.W. (2003). „The enzymes, regulation, and genetics of bile acid synthesis”. Annu. Rev. Biochem. 72: 137-174. PMID 12543708.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.