N1-acetilpoliamin oksidaza
(Preusmjereno sa stranice HPAO-1)
N1-acetilpoliamin oksidaza (EC 1.5.3.13, hPAO-1, PAO (nespecifična), mPAO, hPAO, poliaminska oksidaza (nespecifična)) je enzim sa sistematskim imenom N1-acetilpoliamin:kiseonik oksidoreduktaza (formira 3-acetamidopropanal).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| N1-acetilpoliamin oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.5.3.13 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) N1-acetilspermidin + O2 +H2O putrescin + 3-acetamidopropanal +H2O2
- (2) N1-acetilspermin + O2 +H2O spermidin + 3-acetamidopropanal +H2O2
Ovaj enzim takođe katalizuje reakcija: N1,N12-diacetilspermin + O2 + H2O = N1-acetilspermidin + 3-acetamamidopropanal + H2O2 On u veoma maloj meri deluje na spermin, ili spermidin u odsustvu aldehida. U prisustvu aldehida enzim katalizuje reakcije:
i sa slabom efikasnošću
- spermidin + O2 + H2O putrescin + 3-aminopropanal + H2</sib>O2
Reference uredi
- ↑ Vujcic, S., Liang, P., Diegelman, P., Kramer, D.L. and Porter, C.W. (2003). „Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion”. Biochem. J. 370: 19-28. PMID 12477380.
- ↑ Jarvinen, A., Grigorenko, N., Khomutov, A.R., Hyvonen, M.T., Uimari, A., Vepsalainen, J., Sinervirta, R., Keinanen, T.A., Vujcic, S., Alhonen, L., Porter, C.W. and Janne, J. (2005). „Metabolic stability of α-methylated polyamine derivatives and their use as substitutes for the natural polyamines”. J. Biol. Chem. 280: 6595-6601. PMID 15611107.
- ↑ Wang, Y., Hacker, A., Murray-Stewart, T., Frydman, B., Valasinas, A., Fraser, A.V., Woster, P.M. and Casero, R.A., Jr. (2005). „Properties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity”. Cancer Chemother. Pharmacol. 56: 83-90. PMID 15791459.
- ↑ Wu, T., Yankovskaya, V. and McIntire, W.S. (2003). „Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N1-acetylated polyamine oxidase”. J. Biol. Chem. 278: 20514-20525. PMID 12660232.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.