Inducibilni faktor hipoksije-prolin dioksigenaza
(Preusmjereno sa stranice HIF hydroxylase)
Inducibilni faktor hipoksije-prolin dioksigenaza (EC 1.14.11.29, HIF hidroksilaza) je enzim sa sistematskim imenom inducibilni faktor hipoksije-L-prolin, 2-oksoglutarat:kiseonik oksidoreduktaza (4-hidroksilacija).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Inducibilni faktor hipoksije-prolin dioksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.11.29 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- inducibilni faktor hipoksije-L-prolin + 2-oksoglutarat + O2 inducibilni faktor hipoksije-trans-4-hidroksi-L-prolin + sukcinat + CO2
Ovaj enzim sadrži gvožđe. Za njegov rad je neophodan askorbat. On specifično vrši hidroksilaciju prolinskog ostataka u HIF-alfa, alfa podjedinici transkripcionog regulatora HIF (inducibilnog faktora hipoksije).
Reference uredi
- ↑ Jaakkola, P., Mole, D.R., Tian, Y.M., Wilson, M.I., Gielbert, J., Gaskell, S.J., Kriegsheim Av, Hebestreit, H.F., Mukherji, M., Schofield, C.J., Maxwell, P.H., Pugh, C.W. and Ratcliffe, P.J. (2001). „Targeting of HIF-α to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation”. Science 292: 468-472. PMID 11292861.
- ↑ Ivan, M., Kondo, K., Yang, H., Kim, W., Valiando, J., Ohh, M., Salic, A., Asara, J.M., Lane, W.S. and Kaelin , W.G., Jr. (2001). „HIFα targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing”. Science 292: 464-468. PMID 11292862.
- ↑ Bruick, R.K. and McKnight, S.L. (2001). „A conserved family of prolyl-4-hydroxylases that modify HIF”. Science 294: 1337-1340. PMID 11598268.
- ↑ Epstein, A.C., Gleadle, J.M., McNeill, L.A., Hewitson, K.S., O'Rourke, J., Mole, D.R., Mukherji, M., Metzen, E., Wilson, M.I., Dhanda, A., Tian, Y.M., Masson, N., Hamilton, D.L., Jaakkola, P., Barstead, R., Hodgkin, J., Maxwell, P.H., Pugh, C.W., Schofield, C.J. and Ratcliffe, P.J. (2001). „C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation”. Cell 107: 43-54. PMID 11595184.
- ↑ Oehme, F., Ellinghaus, P., Kolkhof, P., Smith, T.J., Ramakrishnan, S., Hutter, J., Schramm, M. and Flamme, I. (2002). „Overexpression of PH-4, a novel putative proline 4-hydroxylase, modulates activity of hypoxia-inducible transcription factors”. Biochem. Biophys. Res. Commun. 296: 343-349. PMID 12163023.
- ↑ McNeill, L.A., Hewitson, K.S., Gleadle, J.M., Horsfall, L.E., Oldham, N.J., Maxwell, P.H., Pugh, C.W., Ratcliffe, P.J. and Schofield, C.J. (2002). „The use of dioxygen by HIF prolyl hydroxylase (PHD1)”. Bioorg. Med. Chem. Lett. 12: 1547-1550. PMID 12039559.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.