Fitoen desaturaza (formira neurosporen)
Fitoen desaturaza (formira neurosporen) (EC 1.3.99.28, trostepna fitoenska desaturaza, fitoenska desaturaza (nespecifična), CrtI (nespecifična)) je enzim sa sistematskim imenom 15-cis-fitoen:akceptor oksidoreduktaza (formira neurosporen).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Fitoen desaturaza (formira neurosporen) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.3.99.28 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 15-cis-fitoen + 3 akceptor sve-trans-neurosporen + 3 redukovani akceptor (sveukupna reakcija)
- (1a) 15-cis-fitoen + akceptor sve-trans-fitofluen + redukovani akceptor
- (1b) sve-trans-fitofluen + akceptor sve-trans-zeta-karoten + redukovani akceptor
- (1c) sve-trans-zeta-karoten + akceptor sve-trans-neurosporen + redukovani akceptor
Ovaj enzim učestvuje u karotenoidnoj biosintezi. On katalizuje od jednog do tri koraka desaturacije, cf. EC 1.3.99.29, fitoenska desaturaza (formira zeta-karoten), EC 1.3.99.30, fitoenska desaturaza (formira 3,4-didehidrolikopen), EC 1.3.99.31, fitoenska desaturaza (formira likopen). Ovaj enzim aktivfira FAD.
Reference uredi
- ↑ Raisig, A., Bartley, G., Scolnik, P. and Sandmann, G. (1996). „Purification in an active state and properties of the 3-step phytoene desaturase from Rhodobacter capsulatus overexpressed in Escherichia coli”. J. Biochem. 119: 559-564. PMID 8830054.
- ↑ Wang, C.W. and Liao, J.C. (2001). „Alteration of product specificity of Rhodobacter sphaeroides phytoene desaturase by directed evolution”. J. Biol. Chem. 276: 41161-41164. PMID 11526111.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.