Eritronolid sintaza
Eritronolid sintaza (EC 2.3.1.94, eritronolid kondenzujući enzim, malonil-KoA:propionil-KoA maloniltransferaza (ciklizacija), eritronolidna sintaza, malonil-KoA:propanoil-KoA maloniltransferaza (ciklizacija), dezoksieritronolidna B sintaza, 6-dezoksieritronolidna B sintaza, DEBS) je enzim sa sistematskim imenom propanoil-KoA:(2S)-metilmalonil-KoA maloniltransferaza (ciklizacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Eritronolid sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.3.1.94 | ||||||||
| CAS broj | 87683-77-0 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- propanoil-KoA + 6 (2S)-metilmalonil-KoA + 6 NADPH + 6 H+ 6-dezoksieritronolid B + 7 KoA + 6 CO2 +H2O + 6 NADP+
Produkt, 6-dezoksieritronolid B, sadrži 14-člani laktonski prsten. On je intermedijer u biosintezi eritromicinskih antibiotika.
Reference uredi
- ↑ Omura, S. and Nakagawa, A. (1981). „Biosynthesis of 16-membered macrolide antibiotics”. Antibiotics 4: 175-192.
- ↑ Roberts, G. and Leadley, P.F. (1984). „Use of [3H]tetrahydrocerulenin to assay condensing enzyme activity in Streptomyces erythreus”. Biochem. Soc. Trans. 12: 642-643.
- ↑ Pfeifer, B.A., Admiraal, S.J., Gramajo, H., Cane, D.E. and Khosla, C. (2001). „Biosynthesis of complex polyketides in a metabolically engineered strain of E. coli”. Science 291: 1790-1792. PMID 11230695.
- ↑ Tsai, S.C., Miercke, L.J., Krucinski, J., Gokhale, R., Chen, J.C., Foster, P.G., Cane, D.E., Khosla, C. and Stroud, R.M. (2001). „Crystal structure of the macrocycle-forming thioesterase domain of the erythromycin polyketide synthase: versatility from a unique substrate channel”. Proc. Natl. Acad. Sci. USA 98: 14808-14813. PMID 11752428.
- ↑ Khosla, C., Tang, Y., Chen, A.Y., Schnarr, N.A. and Cane, D.E. (2007). „Structure and mechanism of the 6-deoxyerythronolide B synthase”. Annu. Rev. Biochem. 76: 195-221. PMID 17328673.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.