Eritromicin 12 hidroksilaza
Eritromicin 12 hidroksilaza (EC 1.14.13.154, EryK) je enzim sa sistematskim imenom eritromicin-D,NADPH:kiseonik oksidoreduktaza (12-hidroksilacija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Eritromicin 12 hidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.154 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- eritromicin D + NADPH + H+ + O2 eritromicin C + NADP+ + H2O
Ovaj enzim je odgovoran za C-12 hidroksilaciju makrolaktonskog prstena, jedan od zadnjih koraka biosinteze eritromicina.
Reference uredi
- ↑ Lambalot, R.H., Cane, D.E., Aparicio, J.J. and Katz, L. (1995). „Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK”. Biochemistry 34: 1858-1866. PMID 7849045.
- ↑ Savino, C., Montemiglio, L.C., Sciara, G., Miele, A.E., Kendrew, S.G., Jemth, P., Gianni, S. and Vallone, B. (2009). „Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate”. J. Biol. Chem. 284: 29170-29179. PMID 19625248.
- ↑ Montemiglio, L.C., Gianni, S., Vallone, B. and Savino, C. (2010). „Azole drugs trap cytochrome P450 EryK in alternative conformational states”. Biochemistry 49: 9199-9206. PMID 20845962.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.