3-keto-steroid reduktaza
(Preusmjereno sa stranice ERG27 (gen))
3-keto-steroid reduktaza (EC 1.1.1.270, 3-KSR, HSD17B7, ERG27) je enzim sa sistematskim imenom 3beta-hidroksisteroid:NADP+ 3-oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 3-keto-steroid reduktaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.270 | ||||||||
| CAS broj | 42616-29-5 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 3beta-hidroksisteroid + NADP+ 3-oksosteroid + NADPH + H+
Ovaj enzim deluje na višestruke 3beta-hidroksisteroide. On učestvuje u biosintezi zemosterol i holesterola, pri čemu katalizuje reakciju u suprotnom smeru od prikazanog. Enzim sisara je bifunkcionalan i takođe deluje kao EC 1.1.1.62, 17beta-estradiol 17-dehidrogenaza.
Reference
uredi- ↑ Swindell, A.C. and Gaylor, J.L. (1968). „Investigation of the component reactions of oxidative sterol demethylation. Formation and metabolism of 3-ketosteroid intermediates”. J. Biol. Chem. 243: 5546-5555. PMID 4387005.
- ↑ Billheimer, J.T., Alcorn, M. and Gaylor, J.L. (1981). „Solubilization and partial purification of a microsomal 3-ketosteroid reductase of cholesterol biosynthesis. Purification and properties of 3β-hydroxysteroid dehydrogenase and Δ5-3-ketosteroid isomerase from bovine corpora lutea”. Arch. Biochem. Biophys. 211: 430-438. PMID 6946726.
- ↑ Gachotte, D., Sen, S.E., Eckstein, J., Barbuch, R., Krieger, M., Ray, B.D. and Bard, M. (1999). „Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation”. Proc. Natl. Acad. Sci. USA 96: 12655-12660. PMID 10535978.
- ↑ Marijanovic, Z., Laubner, D., Moller, G., Gege, C., Husen, B., Adamski, J. and Breitling, R. (2003). „Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis”. Mol. Endocrinol. 17: 1715-1725. PMID 12829805.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.