Dihloroarcirijaflavin A sintaza
Dihloroarcirijaflavin A sintaza (EC 1.13.12.17, dihloroarcirijaflavinska A sintaza) je enzim sa sistematskim imenom dihlorohromopirolat,NADH:kiseonik 2,5-oksidoreduktaza (formira dihloroarcirijaflavin A).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Dihloroarcirijaflavin A sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.13.12.17 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
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- dihlorohromopirolat + 4 O2 + 4 NADH + 4 H+ dihloroarcirijaflavin A + 2 CO2 + 6H2O + 4 NAD+
Konverzija dihlorohromopirolata u dihloroarcirijaflavin A je kompleksan process u kome učestvuju dve enzimske komponente. RebP je NAD-zavisna citohrom P450 oksigenaza koja izvodi formiranje aril-aril veze čime se formira šestočlani prsten indolokarbazola. Zajedno sa RebC, flavin-zavisnom hidroksilaza, on takođe katalizuje oksidativnu dekarboksilaciju obe karboksilne grupe.
Reference uredi
- ↑ Makino, M., Sugimoto, H., Shiro, Y., Asamizu, S., Onaka, H. and Nagano, S. (2007). „Crystal structures and catalytic mechanism of cytochrome P450 StaP that produces the indolocarbazole skeleton”. Proc. Natl. Acad. Sci. USA 104: 11591-11596. PMID 17606921.
- ↑ Howard-Jones, A.R. and Walsh, C.T. (2006). „Staurosporine and rebeccamycin aglycones are assembled by the oxidative action of StaP, StaC, and RebC on chromopyrrolic acid”. J. Am. Chem. Soc. 128: 12289-12298. PMID 16967980.
- ↑ Sanchez, C., Zhu, L., Brana, A.F., Salas, A.P., Rohr, J., Mendez, C. and Salas, J.A. (2005). „Combinatorial biosynthesis of antitumor indolocarbazole compounds”. Proc. Natl. Acad. Sci. USA 102: #461-466. PMID 15625109.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.