Diacetil reduktaza (formira (S)-acetoin)
Diacetil reduktaza (formira (S)-acetoin) (EC 1.1.1.304, (S)-acetoinska dehidrogenaza) je enzim sa sistematskim imenom (S)-acetoin:NAD+ oksidoreduktaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Diacetil reduktaza (formira (S)-acetoin) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.304 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (S)-acetoin + NAD+ diacetil + NADH + H+
Ova reakcija je katalizovana u reverznom smeru. Ta aktivnost je obično vezana za dejstvo butandiolne dehidrogenaze (EC 1.1.1.4 ili EC 1.1.1.76). Dok je dejstvo butandiolne dehidrogenaze reverzibilno, dejstvo diacetil reduktaze je ireverzibilno. Ovaj enzim je izražen u Geobacillus steardrugemophilus, Enterobacter aerogenes i Klebsiella pneumoniae.
Reference uredi
- ↑ Giovannini, P.P., Medici, A., Bergamini, C.M. and Rippa, M. (1996). „Properties of diacetyl (acetoin) reductase from Bacillus stearothermophilus”. Bioorg. Med. Chem. 4: 1197-1201. PMID 8879540.
- ↑ Carballo, J., Martin, R., Bernardo, A. and Gonzalez, J. (1991). „Purification, characterization and some properties of diacetyl(acetoin) reductase from Enterobacter aerogenes”. Eur. J. Biochem. 198: 327-332. PMID 2040298.
- ↑ Ui, S., Okajima, Y., Mimura, A., Kanai, H., Kobayashi, T., Kudo, T. (1997). „Sequence analysis of the gene for and characterization of D-acetoin forming meso-2,3-butanediol dehydrogenase of 'Klebsiella pneumoniae' expressed in 'Escherichia coli'”. J. Ferment. Bioeng. 83: 32-37.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.