Dezoksihipuzin sintaza
Dezoksihipuzin sintaza (EC 2.5.1.46, spermidin:eIF5A-lizin 4-aminobutiltransferaza (formira propan-1,3-diamin)) je enzim sa sistematskim imenom (eIF5A-prekurzor)-lizin:spermidin 4-aminobutiltransferaza (formira propan-1,3-diamin).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Dezoksihipuzin sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.5.1.46 | ||||||||
| CAS broj | 127069-31-2 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- [eIF5A-prekurzor]-lizin + spermidin [eIF5A-prekurzor]-dezoksihipuzin + propan-1,3-diamin (sveukupna reakcija)
- (1a) spermidin + NAD+ dehidrospermidin + NADH
- (1b) dehidrospermidin + [enzim]-lizin N-(4-aminobutiliden)-[enzim]-lizin + propan-1,3-diamin
- (1c) N-(4-aminobutiliden)-[enzim]-lizin + [eIF5A-prekurzor]-lizin N-(4-aminobutiliden)-[eIF5A-prekurzor]-lizin + [enzim]-lizin
- (1d) N-(4-aminobutiliden)-[eIF5A-prekurzor]-lizin + NADH + H+ [eIF5A-prekurzor]-dezoksihipuzin + NAD+
Eukariotski inicijacioni faktor eIF5A sadrži hipuzinski ostatak koji je esencijalan za aktivnost. Ovaj enzim katalizuje prvu reakciju formiranja hipuzina.
Reference uredi
- ↑ Wolff, E.C., Park, M.H. and Folk, J.E. (1990). „Cleavage of spermidine as the first step in deoxyhypusine synthesis. The role of NAD+”. J. Biol. Chem. 265: 4793-4799. PMID 1690726.
- ↑ Wolff, E.C., Folk, J.E. and Park, M.H. (1997). „Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase”. J. Biol. Chem. 272: 15865-15871. PMID 9188485.
- ↑ Chen, K.Y. and Liu, A.Y.C. (1997). „Biochemistry and function of hypusine formation on eukaryotic initiation factor 5A”. Biol. Signals 6: 105-109. PMID 9285092.
- ↑ Ober, D. and Hartmann, T. (1999). „Deoxyhypusine synthase from tobacco. cDNA isolation, characterization, and bacterial expression of an enzyme with extended substrate specificity”. J. Biol. Chem. 274: 32040-32047. PMID 10542236.
- ↑ Ober, D. and Hartmann, T. (1999). „Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase”. Proc. Natl. Acad. Sci. USA 96: 14777-14782. PMID 10611289.
- ↑ Wolff, E.C. and Park, M.H. (1999). „Identification of lysine350 of yeast deoxyhypusine synthase as the site of enzyme intermediate formation”. Yeast 15: 43-50. PMID 10028184.
- ↑ Wolff, E.C., Wolff, J. and Park, M.H. (2000). „Deoxyhypusine synthase generates and uses bound NADH in a transient hydride transfer mechanism”. J. Biol. Chem. 275: 9170-9177. PMID 10734052.
- ↑ Joe, Y.A., Wolff, E.C. and Park, M.H. (1995). „Cloning and expression of human deoxyhypusine synthase cDNA: structure-function studies with the recombinant enzyme and mutant proteins”. J. Biol. Chem. 270: 22386-22392. PMID 7673224.
- ↑ Tao, Y. and Chen, K.Y. (1995). „Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA”. J. Biol. Chem. 270: 23984-23987. PMID 7592594.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.