2-Halokiselinska dehalogenaza (invertovanje konfiguracije)
(Preusmjereno sa stranice DL-2-haloacid dehalogenase (inversion of configuration))
2-Halokiselinska dehalogenaza (invertovanje konfiguracije) (EC 3.8.1.10, 2-haloalkanoidno kiselinska dehalogenaza, 2-haloalkanoidno kiselinska halidohidrolaza, DL-2-halokiselinska dehalogenaza, DL-2-halokiselinska dehalogenaza (inverzija konfiguracije), DL-2-halokiselinska halidohidrolaza (inverzija konfiguracije), DL-DEXi, (R,S)-2-halokiselinska dehalogenaza (inverzija konfiguracije)) je enzim sa sistematskim imenom (S)-2-halokiselina dehalogenaza (inverzija konfiguracije).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 2-Halokiselinska dehalogenaza (invertovanje konfiguracije) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.8.1.10 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) (S)-2-halokiselina + H2O (R)-2-hidroksikiselina + halid
- (2) (R)-2-halokiselina + H2O (S)-2-hidroksikiselina + halid
Ovaj enzim dehalogenuje (S)- i (R)-2-haloalkanoinske kiseline do korespondirajućih (R)- i (S)-hidroksialkanoinskih kiselina.
Reference uredi
- ↑ Motosugi, K., Esahi, N. and Soda, K. (1982). „Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase”. Arch. Microbiol. 131: 179-183. PMID 7103659.
- ↑ Motosugi, K., Esahi, N. and Soda, K. Purification and properties of a new enzyme, DL- (1982). „2-haloacid dehalogenase, from Pseudomonas sp”. J. Bacteriol. 150: 522-527. PMID 7068529.
- ↑ Motosugi, K., Esahi, N. and Soda, K. (1984). „Enzymatic preparation of D- and L-lactic acid from racemic 2-chloropropionic acid”. Biotechnol. Bioeng. 26: 805-806. PMID 18889931.
- ↑ Kurihara, T., Esaki, N. and Soda, K. (2000). „Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms”. J. Mol. Catal., B Enzym. 10: 57-65.
- ↑ Liu, J.-Q., Kurihara, T., Hasan, A.K.M.Q., Nardi-Dei, V., Koshikawa, H., Esaki, N. and Soda, K. (1994). „Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL”. Appl. Environ. Microbiol. 60: 2389-2393. PMID 8074519.
- ↑ Cairns, S.S., Cornish, A. and Cooper, R.A. (1996). „Cloning, sequencing and expression in Escherichia coli of two Rhizobium sp. genes encoding haloalkanoate dehalogenases of opposite stereospecificity”. Eur. J. Biochem. 235: 744-749. PMID 8654424.
- ↑ Leigh, J.A., Skinner, A.J. and Cooper, R.A. (1988). „Partial purification, stereospecificity and stoichiometry of three dehalogenases from a Rhizobium species”. FEMS Microbiol. Lett. 49: 353-356.
- ↑ Weightman, A.J., Weightman, A.L. and Slater, J.H. (1982). „Stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of Pseudomonas P3: evidence for two different dehalogenation mechanisms”. J. Gen. Microbiol. 128: 1755-1762. PMID 7142958.
- ↑ Soda, K., Kurihara, T., Liu, J.-Q., Nardi-Dei, V., Park, C., Miyagi, M., Tsunasawa, S. and Esaki, N. (1996). „Bacterial 2-haloacid dehalogenases: Structures and catalytic properties”. Pure Appl. Chem. 68: 2097-2103.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.