4-Hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il glukozidna beta-D-glukozidaza
(Preusmjereno sa stranice DIMBOA glucosidase)
4-Hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il glukozidna beta-D-glukozidaza (EC 3.2.1.182, DIMBOAGlc hidrolaza, DIMBOA glukozidaza) je enzim sa sistematskim imenom (2R)-4-hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il beta-D-glukopiranozid beta-D-glukozidaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 4-Hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il glukozidna beta-D-glukozidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.2.1.182 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) (2R)-4-hidroksi-7-metoksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il beta-D-glukopiranozid + H2O 2,4-dihidroksi-7-metoksi-2H-1,4-benzoksazin-3(4H)-on + D-glukoza
- (2) (2R)-4-hidroksi-3-okso-3,4-dihidro-2H-1,4-benzoksazin-2-il beta-D-glukopiranozid + H2O
2,4-dihidroksi-2H-1,4-benzoksazin-3(4H)-on + D-glukoza
Enzim iz Triticum aestivum (pšenice) ima veći afinitet za DIMBOA glukozid nego za DIBOA glukozid.
Reference uredi
- ↑ Sue, M., Ishihara, A. and Iwamura, H. (2000). „Purification and characterization of a hydroxamic acid glucoside β-glucosidase from wheat (Triticum aestivum L.) seedlings”. Planta 210: 432-438. PMID 10750901.
- ↑ Sue, M., Ishihara, A. and Iwamura, H. (2000). „Purification and characterization of a β-glucosidase from rye (Secale cereale L.) seedlings”. Plant Sci. 155: 67-74. PMID 10773341.
- ↑ Czjzek, M., Cicek, M., Zamboni, V., Bevan, D.R., Henrissat, B. and Esen, A. (2000). „The mechanism of substrate (aglycone) specificity in β-glucosidases is revealed by crystal structures of mutant maize β-glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes”. Proc. Natl. Acad. Sci. USA 97: 13555-13560. PMID 11106394.
- ↑ Nikus, J., Esen, A. and Jonsson, L.M.V. (2003). „Cloning of a plastidic rye (Secale cereale) β-glucosidase cDNA and its expression in Escherichia coli”. Physiol. Plantarum 118: 337-348.
- ↑ Sue, M., Yamazaki, K., Yajima, S., Nomura, T., Matsukawa, T., Iwamura, H. and Miyamoto, T. (2006). „Molecular and structural characterization of hexameric β-D-glucosidases in wheat and rye”. Plant Physiol. 141: 1237-1247. PMID 16751439.
- ↑ Sue, M., Nakamura, C., Miyamoto, T. and Yajima, S. (2011). „Active-site architecture of benzoxazinone-glucoside β-D-glucosidases in Triticeae”. Plant Sci. 180: 268-275. PMID 21421370.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.