D-Man-alfa-(1-3)-D-Glc-beta-(1-4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza

D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza
Identifikatori
EC broj	2.4.1.264
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB	RCSB PDB PDBe PDBj PDBsum
Pretraga
PMC	articles
PubMed	articles
NCBI Protein	search

D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfoundekaprenol 2-beta-glukuroniltransferaza (EC 2.4.1.264, GumK) je enzim sa sistematskim imenom UDP-glukuronat:D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol beta-1,2-glukuroniltransferaza.^[1]^[2]^[3]^[4]^[5]^[6]^[7] Ovaj enzim katalizuje sledeću hemijsku reakciju

UDP-glukuronat + D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol

\rightleftharpoons

UDP + D-GlcA-beta-(1->2)-D-Man-alfa-(1->3)-D-Glc-beta-(1->4)-D-Glc-alfa-1-difosfo-ditrans,oktacis-undekaprenol

Ovaj enzim učestvuje u biosintezi eksopolisaharida ksantana (kod bakterije Xanthomonas campestris) i acetana (kod bakterije Glukonacetobacter xylinus).

Reference uredi

↑ Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Puhler, A. and Ielpi, L. (1998). „Xanthomonas campestris' pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence”. J. Bacteriol. 180: 1607-1617. PMID 9537354.
↑ Ielpi, L., Couso, R.O. and Dankert, M.A. (1993). „Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris”. J. Bacteriol. 175: 2490-2500. PMID 7683019.
↑ Kim, S.Y., Kim, J.G., Lee, B.M. and Cho, J.Y. (2009). „Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae' pv oryzae”. Biotechnol. Lett. 31: 265-270. PMID 18854951.
↑ Barreras, M., Bianchet, M.A. and Ielpi, L. (2006). „Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 880-883. PMID 16946469.
↑ Barreras, M., Salinas, S.R., Abdian, P.L., Kampel, M.A. and Ielpi, L. (2008). „Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase”. J. Biol. Chem. 283: 25027-25035. PMID 18596046.
↑ Vojnov, A.A., Bassi, D.E., Daniels, M.J. and Dankert, M.A. (2002). „Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris”. Carbohydr. Res. 337: 315-326. PMID 11841812.
↑ Barreras, M., Abdian, P.L. and Ielpi, L. (2004). „Functional characterization of GumK, a membrane-associated β-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis”. Glycobiology 14: 233-241. PMID 14736729.

Literatura uredi

Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.

Spoljašnje veze uredi

MeSH >3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol+2-beta-glucuronyltransferase D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol+2-beta-glucuronyltransferase

[1] Katzen, F., Ferreiro, D.U., Oddo, C.G., Ielmini, M.V., Becker, A., Puhler, A. and Ielpi, L. (1998). „Xanthomonas campestris' pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence”. J. Bacteriol. 180: 1607-1617. PMID 9537354.

[2] Ielpi, L., Couso, R.O. and Dankert, M.A. (1993). „Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris”. J. Bacteriol. 175: 2490-2500. PMID 7683019.

[3] Kim, S.Y., Kim, J.G., Lee, B.M. and Cho, J.Y. (2009). „Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae' pv oryzae”. Biotechnol. Lett. 31: 265-270. PMID 18854951.

[4] Barreras, M., Bianchet, M.A. and Ielpi, L. (2006). „Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis”. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 880-883. PMID 16946469.

[5] Barreras, M., Salinas, S.R., Abdian, P.L., Kampel, M.A. and Ielpi, L. (2008). „Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase”. J. Biol. Chem. 283: 25027-25035. PMID 18596046.

[6] Vojnov, A.A., Bassi, D.E., Daniels, M.J. and Dankert, M.A. (2002). „Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris”. Carbohydr. Res. 337: 315-326. PMID 11841812.

[7] Barreras, M., Abdian, P.L. and Ielpi, L. (2004). „Functional characterization of GumK, a membrane-associated β-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis”. Glycobiology 14: 233-241. PMID 14736729.

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