Cijanoficinaza
(Preusmjereno sa stranice Cyanophycinase)
Cijanoficinaza (EC 3.4.15.6, cijanoficin degradirajući enzim, beta-Asp-Arg hidrolizujući enzim, CGPaza, CphB, CphE, cijanoficin granularna polipeptidaza, ekstracelularna CGPaza) je enzim.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Cijanoficinaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.15.6 | ||||||||
| CAS broj | 131554-16-0 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- [L-Asp(4-L-Arg)]n + H2O [L-Asp(4-L-Arg)]n-1 + L-Asp(4--L-Arg)
Ovaj enzim je visoko specifičan za razgranate polipeptidne cijanoficine i ne hidrolizuje poli-L-aspartat ili poli-L-arginin.
Reference uredi
- ↑ Obst, M., Krug, A., Luftmann, H. and Steinbüchel, A. (2005). „Degradation of cyanophycin by Sedimentibacter hongkongensis strain KI and Citrobacter amalonaticus strain G isolated from an anaerobic bacterial consortium”. Appl. Environ. Microbiol. 71: 3642-3652. PMID 16000772.
- ↑ Obst, M., Oppermann-Sanio, F.B., Luftmann, H. and Steinbüchel, A. (2002). „Isolation of cyanophycin-degrading bacteria, cloning and characterization of an extracellular cyanophycinase gene (cphE) from Pseudomonas anguilliseptica strain BI. The cphE gene from P. anguilliseptica BI encodes a cyanophycin-hydrolyzing enzyme”. J. Biol. Chem. 277: 25096-25105. PMID 11986309.
- ↑ Richter, R., Hejazi, M., Kraft, R., Ziegler, K. and Lockau, W. (1999). „Cyanophycinase, a peptidase degrading the cyanobacterial reserve material multi-L-arginyl-poly-L-aspartic acid (cyanophycin): molecular cloning of the gene of Synechocystis sp. PCC 6803, expression in Escherichia coli, and biochemical characterization of the purified enzyme”. Eur. J. Biochem. 263: 163-169. PMID 10429200.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.