Amorfa-4,11-dien 12-monooksigenaza
(Preusmjereno sa stranice CYP71AV1)
Amorfa-4,11-dien 12-monooksigenaza (EC 1.14.13.158, CYP71AV1) je enzim sa sistematskim imenom amorfa-4,11-dien,NADPH:kiseonik oksidoreduktaza (12-hidroksilacija).[1] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Amorfa-4,11-dien 12-monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.158 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- amorfa-4,11-dien + 3 O2 + 3 NADPH + 3 H+ artemisinat + 3 NADP+ + 4H2O (sveukupna reakcija)
- (1a) amorfa-4,11-dien + O2 + NADPH + H+ artemisinski alkohol + NADP+ + H2O
- (1b) artemisinski alkohol + O2 + NADPH + H+ artemisinski aldehid + NADP+ + 2H2O
- (1c) artemisinski aldehid + O2 + NADPH + H+ artemisinat + NADP+ + H2O
Ovaj enzim je hem-tiolatni protein (P-450). On je kloniran iz biljke Artemisia annua.
Reference
uredi- ↑ Teoh, K.H., Polichuk, D.R., Reed, D.W., Nowak, G. and Covello, P.S. (2006). „Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin”. FEBS Lett. 580: 1411-1416. PMID 16458889.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.