Holest-4-en-3-on 26-monooksigenaza
(Preusmjereno sa stranice CYP125A1)
Holest-4-en-3-on 26-monooksigenaza (EC 1.14.13.141, CYP125, CYP125A1, holest-4-en-3-on 27-monooksigenaza) je enzim sa sistematskim imenom holest-4-en-3-on,NADH:kiseonik oksidoreduktaza (26-hidroksilacija).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Holest-4-en-3-on 26-monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.141 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- holest-4-en-3-on + NADH + H+ + O2 26-hidroksiholest-4-en-3-on + NAD+ + H2O
Ovaj hem tiolatni (P450) enzim, prisutan u nekoliko bakterijskih patogena, učestvuje u degradaciji domaćinovog holesterola. On katalizuje hidroksilaciju na C-26, čemu sledi oksidacija alkohola do karboksilne kiseline putem aldehidnog intermedijera.
Reference uredi
- ↑ Rosloniec, K.Z., Wilbrink, M.H., Capyk, J.K., Mohn, W.W., Ostendorf, M., van der Geize, R., Dijkhuizen, L. and Eltis, L.D. (2009). „Cytochrome P450 125 (CYP125) catalyses C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1”. Mol. Microbiol. 74: 1031-1043. PMID 19843222.
- ↑ McLean, K.J., Lafite, P., Levy, C., Cheesman, M.R., Mast, N., Pikuleva, I.A., Leys, D. and Munro, A.W. (2009). „The Structure of Mycobacterium tuberculosis CYP125: molecular basis for cholesterol binding in a P450 needed for host infection”. J. Biol. Chem. 284: 35524-35533. PMID 19846552.
- ↑ Capyk, J.K., Kalscheuer, R., Stewart, G.R., Liu, J., Kwon, H., Zhao, R., Okamoto, S., Jacobs, W.R., Jr., Eltis, L.D. and Mohn, W.W. (2009). „Mycobacterial cytochrome P450 125 (cyp125) catalyzes the terminal hydroxylation of C27 steroids”. J. Biol. Chem. 284: 35534-35542. PMID 19846551.
- ↑ Ouellet, H., Guan, S., Johnston, J.B., Chow, E.D., Kells, P.M., Burlingame, A.L., Cox, J.S., Podust, L.M. and de Montellano, P.R. (2010). „Mycobacterium tuberculosis CYP125A1, a steroid C27 monooxygenase that detoxifies intracellularly generated cholest-4-en-3-one”. Mol. Microbiol. 77: 730-742. PMID 20545858.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.