Steroid 15beta-monooksigenaza
(Preusmjereno sa stranice CYP106A2)
Steroid 15beta-monooksigenaza (EC 1.14.15.8, citohrom P-450meg, citohrom P450meg, steroidna 15beta-hidroksilaza, CYP106A2, BmCYP106A2) je enzim sa sistematskim imenom progesteron,redukovani-feredoksin:kiseonik oksidoreduktaza (15beta-hidroksilacija).[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Steroid 15beta-monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.15.8 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- progesteron + redukovani feredoksin + O2 15beta-hidroksiprogesteron + oksidovani feredoksin + H2O
Enzim iz Bacillus megaterium vrši hidroksilaciju raznih 3-okso-Delta4-steroida u poziciji 15beta.
Reference uredi
- ↑ Berg, A., Ingelman-Sundberg, M. and Gustafsson, J.A. (1979). „Purification and characterization of cytochrome P-450meg”. J. Biol. Chem. 254: 5264-5271. PMID 109432.
- ↑ Berg, A., Gustafsson, J.A. and Ingelman-Sundberg, M. (1976). „Characterization of a cytochrome P-450-dependent steroid hydroxylase system present in Bacillus megaterium”. J. Biol. Chem. 251: 2831-2838. PMID 177422.
- ↑ Lisurek, M., Kang, M.J., Hartmann, R.W. and Bernhardt, R. (2004). „Identification of monohydroxy progesterones produced by CYP106A2 using comparative HPLC and electrospray ionisation collision-induced dissociation mass spectrometry”. Biochem. Biophys. Res. Commun. 319: 677-682. PMID 15178459.
- ↑ Goni, G., Zollner, A., Lisurek, M., Velazquez-Campoy, A., Pinto, S., Gomez-Moreno, C., Hannemann, F., Bernhardt, R. and Medina, M. (2009). „Cyanobacterial electron carrier proteins as electron donors to CYP106A2 from Bacillus megaterium ATCC 13368”. Biochim. Biophys. Acta 1794: 1635-1642. PMID 19635596.
- ↑ Lisurek, M., Simgen, B., Antes, I. and Bernhardt, R. (2008). „Theoretical and experimental evaluation of a CYP106A2 low homology model and production of mutants with changed activity and selectivity of hydroxylation”. Chembiochem 9: 1439-1449. PMID 18481342.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.