2-Dezoksi-scilo-inosozna sintaza
(Preusmjereno sa stranice BtrC (gene name))
2-Dezoksi-scilo-inosozna sintaza (EC 4.2.3.124, btrC (gen), neoC (gen), kanC (gen)) je enzim sa sistematskim imenom D-glukoza-6-fosfat fosfat-lijaza (formira 2-dezoksi-scilo-inosoza).[1][2][3][4][5][6][7] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 2-Dezoksi-scilo-inosozna sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.3.124 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- D-glukoza 6-fosfat 2-dezoksi-L-scilo-inosoza + fosfat
Za dejstvo ovog enzima je neophodan jon Co2+.
Reference
uredi- ↑ Kudo, F., Yamauchi, N., Suzuki, R. and Kakinuma, K. (1997). „Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans”. J. Antibiot. (Tokyo) 50: 424-428. PMID 9207913.
- ↑ Kudo, F., Hosomi, Y., Tamegai, H. and Kakinuma, K. (1999). „Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans. A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics”. J. Antibiot. (Tokyo) 52: 81-88. PMID 10344560.
- ↑ Kudo, F., Tamegai, H., Fujiwara, T., Tagami, U., Hirayama, K. and Kakinuma, K. (1999). „Molecular cloning of the gene for the key carbocycle-forming enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics and its comparison with dehydroquinate synthase”. J. Antibiot. (Tokyo) 52: 559-571. PMID 10470681.
- ↑ Huang, Z., Kakinuma, K. and Eguchi, T. (2005). „Stereospecificity of hydride transfer in NAD+-catalyzed 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics”. Bioorg. Chem. 33: 82-89. PMID 15788164.
- ↑ Thuy, M.L., Kharel, M.K., Lamichhane, R., Lee, H.C., Suh, J.W., Liou, K. and Sohng, J.K. (2005). „Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans”. Biotechnol. Lett. 27: 465-470. PMID 15928851.
- ↑ Tamegai, H., Nango, E., Koike-Takeshita, A., Kudo, F. and Kakinuma, K. (2002). „Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans”. Biosci. Biotechnol. Biochem. 66: 1538-1545. PMID 12224638.
- ↑ Tamegai, H., Sawada, H., Nango, E., Aoki, R., Hirakawa, H., Iino, T. and Eguchi, T. (2010). „Roles of a 20 kDa protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism”. Biosci. Biotechnol. Biochem. 74: 1215-1219. PMID 20530911.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.