7,8-Didemetil-8-hidroksi-5-deazariboflavin sintaza
7,8-Didemetil-8-hidroksi-5-deazariboflavin sintaza (EC 2.5.1.77, FO sintaza) je enzim sa sistematskim imenom 5-amino-6-(D-ribitilamino)uracil:4-hidroksifenilpiruvat, 4-metilfenol transferaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 7,8-Didemetil-8-hidroksi-5-deazariboflavin sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.5.1.77 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 5-amino-6-(D-ribitilamino)uracil + 3-(4-hidroksifenil)piruvat + 2 S-adenozil-L-metionin +H2O 7,8-didemetil-8-hidroksi-5-deazariboflavin + 2 L-metionin + 2 5'-dezoksiadenozin + oksalat + NH3
Ovaj enzim sadrži 4Fe-4S klaster. Taj klaster je koordiniran sa 3 cisteina i jednim SAM molekulom.
Reference
uredi- ↑ Graham, D.E., Xu, H. and White, R.H. (2003). „Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F420 biosynthesis”. Arch. Microbiol. 180: 455-464. PMID 14593448.
- ↑ Choi, K.P., Kendrick, N. and Daniels, L. (2002). „Demonstration that fbiC is required by Mycobacterium bovis BCG for coenzyme F420 and FO biosynthesis”. J. Bacteriol. 184: 2420-2428. PMID 11948155.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.