4-Hidroksi-3-metilbut-2-en-1-il difosfat sintaza
4-hidroksi-3-metilbut-2-en-1-il difosfat sintaza (EC 1.17.7.1, 4-hidroksi-3-metilbut-2-en-1-il difosfatna sintaza, (E)-4-hidroksi-3-metilbut-2-en-1-il-difosfat:protein-disulfidna oksidoreduktaza (hidratacija), (E)-4-hidroksi-3-metilbut-2-enil difosfatna sintaza, GcpE) je enzim sa sistematskim imenom (E)-4-hidroksi-3-metilbut-2-en-1-il-difosfat:oksidovani feredoksin oksidoreduktaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 4-hidroksi-3-metilbut-2-en-1-il difosfat sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.17.7.1 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (E)-4-hidroksi-3-metilbut-2-en-1-il difosfat + H2O + 2 oksidovani feredoksin 2-C-metil-D-eritritol 2,4-ciklodifosfat + 2 redukovani feredoksin
Ovaj enzim je gvožđe-sumporni protein koji sadrži [4Fe-4S] kluster. On deluje u reverznom smeru.
Reference
uredi- ↑ Hecht, S., Eisenreich, W., Adam, P., Amslinger, S., Kis, K., Bacher, A., Arigoni, D. and Rohdich, F. (2001). „Studies on the nonmevalonate pathway to terpenes: the role of the GcpE (IspG) protein”. Proc. Natl. Acad. Sci. USA 98: 14837-14842. PMID 11752431.
- ↑ Okada, K. and Hase, T. (2005). „Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1”. J. Biol. Chem. 280: 20672-20679. PMID 15792953.
- ↑ Seemann, M., Wegner, P., Schünemann, V., Tse Sum Bui, B., Wolff, M., Marquet, A., Trautwein, A.X. and Rohmer, M. (2005). „Isoprenoid biosynthesis in chloroplasts via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) from Arabidopsis thaliana is a [4Fe-4S] protein”. J. Biol. Inorg. Chem. 10: 131-137. PMID 15650872.
- ↑ Seemann, M., Tse Sum Bui, B., Wolff, M., Tritsch, D., Campos, N., Boronat, A., Marquet, A. and Rohmer, M. (2002). „Isoprenoid biosynthesis through the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (GcpE) is a [4Fe-4S] protein”. Angew. Chem. Int. Ed. Engl. 41: 4337-4339. PMID 12434382.
- ↑ Seemann, M., Tse Sum Bui, B., Wolff, M., Miginiac-Maslow, M. and Rohmer, M. (2006). „Isoprenoid biosynthesis in plant chloroplasts via the MEP pathway: direct thylakoid/ferredoxin-dependent photoreduction of GcpE/IspG”. FEBS Lett. 580: 1547-1552. PMID 16480720.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.