Kortizon b-reduktaza
Kortizon b-reduktaza (EC 1.3.1.3, 3-okso-Delta4-steroid 5beta-reduktaza, 5beta-reduktaza, androstendionska 5beta-reduktaza, holestenonska 5beta-reduktaza, kortizonska 5beta-reduktaza, kortizonska beta-reduktaza, kortizonska Delta4-5beta-reduktaza, steroidna 5beta-reduktaza, testosteronska 5beta-reduktaza, Delta4-3-ketosteroidna 5beta-reduktaza, Delta4-5beta-reduktaza, Delta4-hidrogenaza, 4,5beta-dihidrokortizon:NADP+ Delta4-oksidoreduktaza, 3-okso-5beta-steroid:NADP+ Delta4-oksidoreduktaza) je enzim sa sistematskim imenom 5beta-holestan-3-on:NADP+ 4,5-oksidoreduktaza.[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Kortizon b-reduktaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.3.1.3 | ||||||||
| CAS broj | 2604034 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) 5beta-holestan-3-on + NADP+ holest-4-en-3-on + NADPH + H+
- (2) 17,21-dihidroksi-5beta-pregnan-3,11,20-trion + NADP+ kortizon + NADPH + H+
Ovaj ljudski enzim efikasno katalizuje redukciju progesterona, androstendiona, 17alfa-hidroksiprogesterona i testosteron do 5beta-redukovanih metabolita; on takođe deluje na aldosteron, kortikosteron i kortizol, mada u manjoj meri. Intermedijeri žučne kiseline 7alfa,12alfa-dihidroksi-4-holesten-3-on i 7alfa-hidroksi-4-holesten-3-on takođe mogu da budu supstrat.
Reference uredi
- ↑ Forchielli, E. and Dorfman, R.I. (1956). „Separation of Δ4-5α- and Δ4-5β-hydrogenases from rat liver homogenates”. J. Biol. Chem. 223: 443-448. PMID 13376613.
- ↑ Brown-Grant, K., Forchielli, E. and Dorfman, R.I. (1960). „The Δ4-hydrogenases of guinea pig adrenal gland”. J. Biol. Chem. 235: 1317-1320. PMID 13805063.
- ↑ Levy, H.R. and Talalay, P. (1957). „Enzymatic introduction of double bonds into steroid ring A”. J. Am. Chem. Soc. 79: 2658-2659.
- ↑ Tomkins, G.M. (1957). „The enzymatic reduction of Δ4-3-ketosteroids”. J. Biol. Chem. 225: 13-24. PMID 13416214.
- ↑ Sugimoto, Y., Yoshida, M. and Tamaoki, B. (1990). „Purification of 5β-reductase from hepatic cytosol fraction of chicken”. J. Steroid Biochem. 37: 717-724. PMID 2278855.
- ↑ Furuebisu, M., Deguchi, S. and Okuda, K. (1987). „Identification of cortisone 5β-reductase as Δ4-3-ketosteroid 5β-reductase”. Biochim. Biophys. Acta 912: 110-114. PMID 3828348.
- ↑ Okuda, A. and Okuda, K. (1984). „Purification and characterization of Δ4-3-ketosteroid 5β-reductase”. J. Biol. Chem. 259: 7519-7524. PMID 6736016.
- ↑ Charbonneau, A. and The, V.L. (2001). „Genomic organization of a human 5β-reductase and its pseudogene and substrate selectivity of the expressed enzyme”. Biochim. Biophys. Acta 1517: 228-235. PMID 11342103.
- ↑ Kondo, K.H., Kai, M.H., Setoguchi, Y., Eggertsen, G., Sjöblom, P., Setoguchi, T., Okuda, K.I. and Björkhem, I. (1994). „Cloning and expression of cDNA of human Δ4-3-oxosteroid 5β-reductase and substrate specificity of the expressed enzyme”. Eur. J. Biochem. 219: 357-363. PMID 7508385.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.