23S rRNK (guanin2535-N1)-metiltransferaza
23S rRNK (guanin2535-N1)-metiltransferaza (EC 2.1.1.209, AviRa) je enzim sa sistematskim imenom S-adenozil-L-metionin:23S rRNK (guanin2535-N1)-metiltransferaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 23S rRNK (guanin2535-N1)-metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.209 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- S-adenozil-L-metionin + guanin2535 u 23S rRNK S-adenozil-L-homocistein + N1-metilguanin2535 u 23S rRNK
Streptomyces viridochromogenes proizvodi antibiotik avilamicin A, koji se vezuje za 50S ribozomalnu podjedinicu i inhibira sintezu proteina. Da bi se zaštitila od antibiotika, Streptomyces viridochromogenes koristi dve metiltransferaze, 23S rRNK (guanin2535-N1)-metiltransferazu i EC 2.1.1.208, 23S rRNK (uridin2479-2-O)-metiltransferazu.
Reference uredi
- ↑ Treede, I., Jakobsen, L., Kirpekar, F., Vester, B., Weitnauer, G., Bechthold, A. and Douthwaite, S. (2003). „The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose”. Mol. Microbiol. 49: 309-318. PMID 12828631.
- ↑ Weitnauer, G., Gaisser, S., Trefzer, A., Stockert, S., Westrich, L., Quiros, L.M., Mendez, C., Salas, J.A. and Bechthold, A. (2001). „An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57”. Antimicrob. Agents Chemother. 45: 690-695. PMID 11181344.
- ↑ Mosbacher, T.G., Bechthold, A. and Schulz, G.E. (2003). „Crystal structure of the avilamycin resistance-conferring methyltransferase AviRa from Streptomyces viridochromogenes”. J. Mol. Biol. 329: 147-157. PMID 12742024.
Literatura uredi
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- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
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