2-Poliprenil-6-hidroksifenil metilaza
2-Poliprenil-6-hidroksifenil metilaza (EC 2.1.1.222, ubiG (gen), ubiG metiltransferaza, 2-oktaprenil-6-hidroksifenil metilaza) je enzim sa sistematskim imenom S-adenozil-L-metionin:3-(sve-trans-poliprenil)benzen-1,2-diol 2-O-metiltransferaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 2-Poliprenil-6-hidroksifenil metilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.222 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- S-adenozil-L-metionin + 3-(sve-trans-poliprenil)benzen-1,2-diol S-adenozil-L-homocistein + 2-metoksi-6-(sve-trans-poliprenil)fenol
UbiG katalizuje oba metilaciona koraka u biosintezi ubihinona kod Escherichia coli. Druga metilacija je klasifikovana kao EC 2.1.1.64 (3-demetilubihinol 3-O-metiltransferaza). Kod eukariota Coq3 katalizuje dva metilaciona koraka biosinteze ubihinona.
Reference uredi
- ↑ Poon, W.W., Barkovich, R.J., Hsu, A.Y., Frankel, A., Lee, P.T., Shepherd, J.N., Myles, D.C. and Clarke, C.F. (1999). „Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis”. J. Biol. Chem. 274: 21665-21672. PMID 10419476.
- ↑ Hsu, A.Y., Poon, W.W., Shepherd, J.A., Myles, D.C. and Clarke, C.F. (1996). „Complementation of coq3 mutant yeast by mitochondrial targeting of the Escherichia coli UbiG polypeptide: evidence that UbiG catalyzes both O-methylation steps in ubiquinone biosynthesis”. Biochemistry 35: 9797-9806. PMID 8703953.
Literatura uredi
- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.