2-Hlorobenzoat 1,2-dioksigenaza
2-Hlorobenzoat 1,2-dioksigenaza (EC 1.14.12.13, 2-hlorobenzoatna 1,2-dioksigenaza) je enzim sa sistematskim imenom 2-halobenzoat,NADH:kiseonik oksidoreduktaza (1,2-hidroksilacija, dehalogenating, dekarboksilacija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 2-Hlorobenzoat 1,2-dioksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.12.13 | ||||||||
| CAS broj | 125268-83-9 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 2-halobenzoat + NADH + H+ + O2 katehol + halidni anjon + NAD+ + CO2
Ovaj enzim je izdvojen iz bakterija Burkholderia cepacia 2CBS. On ima široku supstratnu specifičnost. Supstrati su: 2-fluorobenzoat, 2-hlorobenzoat, 2-bromobenzoat, i 2-iodobenzoat.
Reference
uredi- ↑ Fetzner, S., Mueller, R. and Lingens, F. (1989). „Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS”. Biol. Chem. Hoppe-Seyler 370: 1173-1182. PMID 2610934.
- ↑ Fetzner, S., Muller, R. and Lingens, F. (1992). „Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS”. J. Bacteriol. 174: 279-290. PMID 1370284.
- ↑ Haak, B., Fetzner, S. and Lingens, F. (1995). „Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS”. J. Bacteriol. 177: 667-675. PMID 7530709.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.