2-Hidroksi-3-oksoadipat sintaza
2-Hhidroksi-3-oksoadipat sintaza (EC 2.2.1.5, 2-hidroksi-3-oksoadipat glioksilat-lijaza (karboksilacija), alfa-ketoglutarinska-glioksilna karboligaza, oksoglutarat:glioksilat karboligaza) je enzim sa sistematskim imenom 2-oksoglutarat:glioksilat succinaldehidtransferaza (dekarboksilacija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| 2-Hidroksi-3-oksoadipat sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.2.1.5 | ||||||||
| CAS broj | 9054-72-2 | ||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- 2-oksoglutarat + glioksilat 2-hidroksi-3-oksoadipat + CO2
Za rad ovog bakterijskog enzima je neophodan tiamin difosfat. Produkt se dekarboksiluje do 5-hidroksi-4-oksopentanoata.
Reference
uredi- ↑ Schlossberg, M.A., Bloom, R.J., Richert, D.A. and Westerfield, W.W. (1970). „Carboligase activity of α-ketoglutarate dehydrogenase”. Biochemistry 9: 1148-1153. PMID 5418712.
- ↑ Schlossberg, M.A., Richert, D.A., Bloom, R.J. and Westerfield, W.W. (1968). „Isolation and identification of 5-hydroxy-4-ketovaleric acid as a product of α-ketoglutarate: glyoxylate carboligase”. Biochemistry 7: 333-337. PMID 4320439.
- ↑ Stewart, P.R. and Quayle, J.R. (1967). „The synergistic decarboxylation of glyoxalate and 2-oxoglutarate by an enzyme system from pig-liver mitochondria”. Biochem. J. 102: 885-897. PMID 16742506.
Literatura
uredi- Nicholas C. Price, Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third izd.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 izd.). Wiley-Interscience. ISBN 0471205036.
- Branden C, Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN: 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 izd.). Wiley Classics Library. ISBN 0471303097.
- Robert A. Copeland (2013). Evaluation of Enzyme Inhibitors in Drug Discovery: A Guide for Medicinal Chemists and Pharmacologists (2nd izd.). Wiley-Interscience. ISBN 111848813X.
- Gerhard Michal, Dietmar Schomburg (2012). Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology (2nd izd.). Wiley. ISBN 0470146842.